摘要
根据已发表的新城疫病毒（ＮＤＶ）核衣壳蛋白（ＮＰ）基因序列，设计合成１对长度为３２ｍｅｒ的引物。ＲＴ－ＰＣＲ扩增ＮＤＶＦ４８Ｅ８株、Ｕｌｓｔｅｒ株、ＬａＳｏｔａ株的ＮＰ基因，产物经琼脂糖凝胶电泳分析，均呈现１条长１．５ｋｂ左右的特异性带。将Ｆ４８Ｅ８株扩增产物克隆入ｐＵＣ１８载体，经限制性内切酶分析证实为ＮＰ基因。
According to the reported NP gene sequence of NDV, a pair of 32 mer primers was designed and synthesized. Then the NP genes of three NDV strains (F 48 E 8, Ulster, La Sota) were amplified by reverse transcription polymerase chain reaction (RT PCR). The amplified products were analyzed by agarose gel electrophoresis, all appeared a specific fragment about 1.5 kb in length as expected. The RT PCR product of F 48 E 8 strain was cloned into the pUC18, then tested by restriction endonuclease analysis, the result suggested it was the NP gene of NDV F 48 E 8 strain.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1998年第4期325-327,共3页
Chinese Journal of Veterinary Science
基金
江苏省自然科学基金
江苏省教委自然科学基金
