摘要
钙离子在细胞的生命活动中起着重要的作用，参与了细胞运动、肌肉收缩等过程。为了探讨钙离子浓度与瘢痕挛缩的关系，应用近年来发展起来的Ｆｕｒａ２／ＡＭ技术，采用图像分析处理系统，检测了培养的增生性瘢痕、瘢痕疙瘩及正常皮肤的成纤维细胞内的钙离子浓度。结果表明，增生性瘢痕中钙离子浓度明显高于瘢痕疙瘩和正常皮肤，有显著性差异（Ｐ＜０．０１），而后二者则无显著性差异（Ｐ＞０．０５）。认为。
Free calcium ions, as a kind of message transport substance, is important in cellular activity such as cell movement, cell differentiation and cell proliferation. In order to investigate the relationship between free calcium ions and scar contracture, the fibroblasts which originated from hypertrophic scar, keloid and normal skin were used as the experimental target. The fibroblasts from 4th～6th generations of different sources were used; Then the intracellular free calcium ions concentrations were measured respectively by the fluorescent Ca 2+ indicator Fura 2/AM and Image analysis system. The results showed that the level of Ca 2+ in fibroblasts of hypertrophic scar was higher than that in keloid and normal skin (P<0.01). There was no significant difference between the level of Ca 2+ in keloid and in normal skin. The conclusion was that the concentration of intracellular free calcium ions played an important role in the scar contract, but the exact mechanism was still unclear and required further study.
出处
《中国修复重建外科杂志》
CAS
CSCD
1998年第6期329-331,共3页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家自然科学基金
