摘要
目的探讨同型半胱氨酸（ＨＣＹ）及其代谢相关因子和血脂与冠心病发病之间的关系。方法４０例经冠状动脉造影术证实为正常或冠心病患者，应用高效液相色谱分析测定血浆ＨＣＹ，同时测定血浆叶酸、维生素Ｂ１２浓度及血脂水平，并应用ＰＣＲ检测Ｎ５，Ｎ１０亚甲基四氢叶酸还原酶（ＭＴＨＦＲ）基因型。结果（１）冠心病患者血浆ＨＣＹ浓度（１７１±３６μｍｏｌ／Ｌ）高于正常对照组（７６±１２μｍｏｌ／Ｌ），合并有心肌梗死、高血压和有冠心病家族史的患者，其血浆ＨＣＹ浓度更高；（２）血浆ＨＣＹ浓度与血浆叶酸、维生素Ｂ１２浓度呈明显的非线性相关；（３）ＭＴＨＦＲ纯合子突变者，血浆ＨＣＹ浓度高；（４）ＣＨＤ患者血脂与ＨＣＹ水平无明显相关。结论高同型半胱氨酸血症是冠心病的新的独立危险因素。
Objective To identify hyperhomocysteinemia (HHe) as a new and independent risk factor for coronary heart disease (CHD). Methods The association of coronary heart disease (CHD) and lipid, homocysteine (HCY) and the factors related to its metabolisms were examined. The mutation of the 677C→T transition of MTHFR was determined by PCR based assay. Whole blood and plasma folate (FA) and plasma vitamin B 12 (B 12 ), as cofactors of those enzymes, were determined by radioimmunologic assay. Plasma HCY was determined by HPLC. Results Patients with CHD confirmed by coronary angiography had increased plasma HCY concentrations (17.1±3.6 μmol/L、7 6±1.2 μmol/L). In patients with MI, HPT and family history (FH) of CHD, plasma HCY increased significantly. Plasma HCY concentrations had significant non linear inverse relation with plasma FA and B 12 concentrations. Homozygous mutants showed higher plasma HCY concentrations. Patients with CHD had increased serum CHOL and VLDL levels, but plasma HCY concentrations were not correlated with serum lipid levels. Conclusion Hyperhomocysteinemia is an independent risk factor for coronary heart disease.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1998年第11期821-823,共3页
National Medical Journal of China
基金
国家攀登计划资助
关键词
冠心病
血浆
半胱氨酸
Coronary disease Homocysteine
