摘要
采用离子交换色谱法从污染肝素原料中分离出多硫酸化硫酸软骨素(OSCS),建立了分步醋酸纤维素薄膜电泳法分析污染肝素中OSCS含量的方法。结果表明,先以0.05mol/L醋酸钡缓冲液(pH5.0)电泳,再以0.15mol/L醋酸锌缓冲液(pH6.3)电泳,可以将肝素和OSCS完全分开,检出限为0.1g/L;通过灰度积分建立定量校准曲线,相关系数为0.9934,平均回收率为102.1%～106.1%;RSD为4.1%～6.0%。
Oversulfated chondroitin sulfate （OSCS） , a kind of contaminant, which may be associated with allergic response in heparin （HP） injection, was separated from contaminated heparin material by strong ion- exchange chromatography. A two step cellulose acetate membrane electrophoresis was established to detect and analyze the content of OSCS in contaminated heparin. The experimental result showed that, HP and OSCS can be completely separated by 0.05 mol/L barium acetate buffer（pH 5.0） and then 0.15 mol/L zinc acetate buffer（pH 6.3）, and the detection limit is 0.1 g/L. Gray value integration showed good linear relationship between the gray value and amount of OSCS（r = 0. 9934） with average recovery of 102. 1% -106.1% and RSD of 4.1% -6.0%.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2009年第8期1147-1151,共5页
Chinese Journal of Analytical Chemistry
基金
国际合作项目(No.2007DFA30980)
国家海洋863项目(No.2007AA09Z445)资助
关键词
肝素
硫酸软骨素
醋酸纤维素薄膜电泳
Contaminated heparin, oversulfated chondroitin sulfate, cellulose acetate membrane electrophoresis
