摘要
目的了解创伤弧菌的培养特征、生化反应特性和药物敏感性，以便提高临床检出率，降低病死率。方法收集分离自浙江省嘉兴市第二医院临床死亡病例的创伤弧菌3株，对其进行培养鉴定和药物敏感性试验。结果创伤弧菌在血琼脂中培养呈灰暗、不透明、β-溶血菌落。生化反应乳糖、纤维二糖、O／129（10μg）阳性；肌醇、鼠李糖等阴性。药物敏感性试验显示其对环丙沙星、左氧氟沙星、亚胺培南、头孢吡肟、头孢他啶、头孢曲松、复方磺胺甲恶唑、呋喃妥因敏感，对庆大霉素、妥布霉素、氨曲南和头孢唑啉耐药。结论创伤弧菌可从患者的血液、疱液及粪便样本中分离到，快速检测、早期诊断，并及时给予经验性抗菌药物治疗对降低病死率至关重要。
Objective To investigate the cultivation, biochemical features and drug susceptibilities of Vibrio vulnificus. Methods Three strains of Vibrio vulnificus were isolated from fatal patients in the Second Hospital of Jiaxing, Zhejiang province. Cultivation, identification and antibacterial susceptibility test were performed. Results Vibrio vulnificus grew on blood agar as dull-gray, opaque colonies with β- hemolysis. The organism presented positive in lactose, cellobiose fermentation and O/129 （10μg） tests, but lack of inositol and rhamnus. The antibacterial susceptibility tests showed that Vibrio vulnificus strains were sensitive to ciprofloxacin, levofloxacin, imipenem, cefepime, ceftazidime, ceftriaxone, compound sulfamethoxazole and nitrofurantoin, however, resistant to gentamicin, tobramycin, aztreonam and cefazolin. Conclusions Vibrio vulnificus can be isolated from blood, bubbles fluid, and stool. Rapid identification, early diagnosis, and prompt empirical antibacterial therapy are important for reducing the mortality.
出处
《中华临床感染病杂志》
CAS
2009年第5期293-296,共4页
Chinese Journal of Clinical Infectious Diseases
基金
基金项目：2009年嘉兴市第一批科技计划项目（2009AY2047）
关键词
弧菌
创伤
培养
鉴定
药物敏感性试验
Vibrio vulnificus
Cultivation
Identification
Antibacterial susceptibility tests
