摘要
目的:探讨大鼠骨髓间充质干细胞(MSC)体外分离培养及鉴定。方法:应用贴壁筛选法分离培养W istar大鼠髓间充质干细胞,用直接荧光抗体染色法对培养的细胞进行鉴定。结果:体外培养的原代MSC 9d融合,荧光抗体染色CD44、CD105表达阳性,CD14、CD34表达阴性。结论:贴壁筛选法在体外很容易分离培养和扩增MSC,可作为获得MSC的一种有效手段。
Objective：To investigate the methods of isolation,culture and identification of bone marrow mesenchymal stem cell（MSC）in rat.Methods：Rat bone marrow was obtained.The MSC were isolated and purified by using direct anchoring and identified by using direct fluorescence staining.Results：Primary cultured rat MSC reached fusion within 9 days.These cells were positive for CD44,CD105,negative for CD14,CD34 by fluorescence staining.Conclusion：Rat MSC can be isolated,cultured and amplified easily by using direct anchoring,which can be used as an effective method for culturing rat MSC.
出处
《内蒙古医学院学报》
2009年第4期367-372,共6页
Acta Academiae Medicinae Neimongol
基金
吴阶平医学基金(2008)
关键词
间充质干细胞
骨髓
荧光抗体染色
mesenchymal stem cell
bone marrow
fluorescence staining
