摘要
目的:探讨新的凋亡相关基因Survivin蛋白和p27蛋白肿瘤标志物在人肾细胞癌中的表达及临床意义。方法:采用免疫组化SP法分别测定50例肾癌和12例正常肾组织中Survivin蛋白及p27蛋白的表达,并分析其与肾癌临床、病理的关系。结果:Survivin蛋白在肾癌组织中表达阳性率为68%(34/50),而正常组织中未见表达,两者有统计学意义(P<0.01)。肾癌中Survivin蛋白的表达与肾癌的病理分级呈正相关(P<0.05)。而p27蛋白在正常肾组织中的阳性率为83.3%(10/12),在肾癌组织中的阳性率为42%(21/50),两者有统计学意义(P<0.01)。p27蛋白表达与肾癌的分级呈负相关(P<0.01)。结论:Survivin蛋白表达水平与肾癌的临床分期、淋巴结转移密切相关,且与p27蛋白呈负相关。联检Survivin蛋白和p27蛋白有助于提高对肾癌预后的评估,并可作为判定肾癌生物学行为的客观指标。
Objective To explore the expressions and the clinical significance of the apoptosis related gene survivin protein and p27 protein tumor markers in human renal cell carcinoma（RCC）.Methods The expressions of survivin protein and p27 protein were detected with immunohistochemical S-P assay in tumor tissue of 50 patients with RCC and 12 specimens of normal renal tissue.Results Survivin protein expression were 68%（34/50） positive in RCC,but was zero in normal renal tissue（P〈0.01）.There was a positive correlationship between the expression rate of Survivin protein and the histological tumor grades（P〈0.05）.In the normal renal tissue,p27 protein expression rate were 83.3%（10/12）,being significantly higher than that i.e.42%（21/50） in RCC（P〈0.01）.p27 protein expression and the histological tumor grades was negatively correlated（P〈0.01）.Conclusion Survivin protein and p27 protein expression levels were closely correlated with the histological grading as well as clinical stage of RCC.Combined detection of survivin protein and p27 protein may improve the accuracy of outcome prediction of RCC,and can serve as useful objective markers of biological behavior of RCC.
出处
《放射免疫学杂志》
CAS
2009年第5期535-537,共3页
Journal of Radioimmanology
