摘要
目的:观察丙泊酚麻醉后大鼠不同脑区、不同时期cAMP含量的动态变化,探讨cAMP在丙泊酚麻醉中的作用。方法:Sprague-Dawley(SD)大鼠40只,分层随机区组设计分成5组,每组8只,腹腔注射异丙酚100mg/kg。分别在腹腔注射生理盐水10ml/kg(对照组)、翻正反射即将消失时(诱导期组)、翻正反射消失后2min(麻醉期组)、翻正反射恢复即刻(恢复期组)和翻正反射恢复后1h(清醒期组)断头取脑。放射免疫法测定大脑皮质、海马和脑干的cAMP含量。结果:与对照组比较,诱导期组皮质和麻醉期组皮质、海马、脑干cAMP含量升高。与麻醉期组比较,恢复期组皮质和清醒期组皮质、海马、脑干cAMP含量降低(P<0.05或0.01)。结论:cAMP在丙泊酚的全麻机制中可能发挥重要作用。
Objective： To investigate the effects of propofol on cyclic adenosine monophosphate（cAMP） content in cerebrocortex, hippocampus and brain stem of rats,and to examine the role of cAMP in the mechanism of sevoflurane anesthesia.Methods： Fourty SD rats were delaminately designed and allocated randomly, to 5 groups paunch inject 100mg/kg propofoh paunch inject 0.9% NaCl 10mg/kg（control group, n = 8）, rats＇ righting reflex was about to lose（ induction groupA, n = 8; induction group B, n = 8）, after losing of righting reflex for one minitue（anesthesia groupA, n = 8; anesthesia groupB, n = 8）, righting reflex was about to recover（recovery groupA, n = 8; recovery groupB, n = 8） and one hour after righting reflex recovery （aware groupA, n = 8; aware groupB, n = 8）. The brain tissues were rapidly dissected into eerebrocortex and hippocampus and brain stem. Then the cAMP content was assessed. Results： Compared with the control group, the cAMP content in the cerebrocortex, hippocampus and brain stem of induction and anesthesia group were increased; ompared with the anesthesia group, the cAMP content in the cerebrocortex, hippocampus and brain stem of recovery group and aware group were decreased（ （ P 〈 0.05 orP 〈 0.01 ） ;Conclusion： cAMP may play a important role in the mechanism of sevoflurane anesthesia.
出处
《中国民康医学》
2009年第20期2463-2463,2478,共2页
Medical Journal of Chinese People’s Health
关键词
丙泊酚
脑
环磷腺苷
麻醉
Propofol
Brain
Cycli adenosine monophosphate
Anesthesia
