摘要
目的:寻求在微通道化学芯片上采用流动注射化学发光分析法快速测定临床样品中尿酸含量。方法:自制的微通道化学芯片,在选定实验条件下,利用尿酸对亚铁氰化钾-铁氰化钾-鲁米诺化学发光体系的增强作用实现血清中尿酸的分析。结果:尿酸浓度在2.0×10-5～1.0×10-2g/L范围内与发光强度增加值线性关系良好;检测限(3σ)为4.6×10-7g/L;精密度试验的RSD为3.09%;加标回收率分别为94.0%、99.7%、107.8%。结论:微通道化学芯片制作方法简单,对血清尿酸含量的流动注射测定灵敏度高,简便快速。
Objective：To establish a flow injection chemiluminescence determination method with micropipe-chip for speedy determination of uric acid in clinical samples.Methods：Basing on the rule that uric acid can greatly enhance the chemiluminescence emission intensity of K4Fe（CN）6-K3Fe（CN）6-luminol system,the prepared micropipe-chip was used to analyze the uric acid in serum under the selective conditions.Results：The enhancing chemiluminescence intensity was proportional to uric acid concentration over a range 2.0×10-5-1.0×10-2 g/L.The detection limit（3σ） was 4.6×10-7 g/L.The relative standard deviation（RSD） was 3.09% and the recovery test results were 94.0%,99.7%,and 107.8%.Conclusion：The manufacture technique of micropipr-chip is simple but the proposed determination method of uric acid in human serum is sensitive,fast and convenient.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2009年第8期1063-1065,共3页
Journal of Chongqing Medical University
基金
重庆医科大学校办课题(XB2004-06)
