摘要
目的:探讨苯丙素甙化合物逆转大肠癌耐药与凋亡的关系.方法:实验分3组:LoVo/Adr细胞组(空白对照组);LoVo/Adr细胞+PPG40mg/L组(实验组);LoVo/Adr细胞+VP5mg/L(阳性对照组).采用MTT法检测苯丙素甙对LoVo/Adr细胞的耐药性;流式细胞仪分析苯丙素甙对大肠癌耐药LoVo细胞的凋亡的作用;通过检测pNA量观察苯丙素甙对LoVo/Adr细胞Caspase-3的活性.结果:苯丙素甙可降低阿霉素对LoVo/Adr细胞的IC50值,具有逆转作用,其逆转倍数为9.93;他还可引起LoVo/Adr细胞凋亡,与对照组相比,具有显著性差异(P<0.01).经苯丙素甙作用后,LoVo/Adr细胞pNA释放量明显增加(31.75±4.34pmol/minvs18.45±2.39pmol/min,P<0.01),而同时加入Caspase-3抑制剂Z-VAD-FMK,pNA释放量明显低于实验组(17.69±2.68pmol/minvs31.75±4.34pmol/min,P<0.01).结论:苯丙素甙对耐药LoVo/Adr细胞具有逆转耐药作用,通过Caspase-3途径诱导凋亡可能为其逆转耐药的机制之一.
AIM： To investigate the relationship between the reversing effect of phenylpropanoid glycoside （PPG） on multidrug resistance of colon carcinoma LoVo/Adr cells and apoptosis. METHODS： LoVo/Adr cells were divided into three groups： non-treatment （negative control） group, PPG treatment group （treated with 40 mg/L PPG） and verapamil treatment （positive control） group （treated with 5 mg/L VP）. The effects of PPG on multidrug resistance of LoVo/ Adr cells were examined by MTT （3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide） assay. The effects of PPG on cell＇apoptosis were detected by flow cytometry. The effects of PPG on the activity of Caspase-3 were evaluated by determining pNA release rate.RESULTS： PPG could decrease the half maximal inhibitory concentration （ICs0） of adriamycin in LoVo cells and reverse their resistance to adriamycin. The reversal index was 9.93. PPG could significantly induce the apoptosis of LoVo cells when compared with the non-treatment group （P 〈 0.01）. The rate of pNA release in the PPG treatment group was significantly higher than that in the non-treatment group （31.75± 4.34 pmol/min vs 18.45 ±2.39 pmol/min, P 〈 0.01）. Caspase-3 inhibitor Z-VAD-FMK could significantly inhibit PPG-induced pNA release （17.69 ±2.68 pmol/min vs 31.75± 4.34 pmol/min, P 〈 0.01）. CONCLUSION： PPG reverse multidrug resistance of LoVo/Adr cells perhaps through induction of Caspase 3-dependent apoptosis.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第23期2357-2361,共5页
World Chinese Journal of Digestology
关键词
苯丙素甙
多药耐药
逆转
凋亡
Phenylpropanoid.glycoside
Multidrug resistance
Reverse
Apoptosis
