摘要
目的:观察脂联素在小鼠急性肝损伤的表达及作用.方法:健康♂BALB/c小鼠随机分为3组.刀豆蛋白A(concanavalin A,ConA)组8只:ConA30mg/kg尾静脉注射.脂联素组5只:在静脉注射ConA前12h和2h,分别应用脂联素3mg/kg腹膜内注射.对照组8只:尾静脉注射生理盐水10mL/kg.注射ConA8h后处死小鼠,检测脂联素及ALT血清水平、肝组织病理变化、肝组织脂联素蛋白及mRNA的表达.结果:脂联素血清水平在脂联素组与对照组无差异,2组均高于ConA组(15.4±3.0mg/L,16.5±2.8mg/Lvs11.8±2.1mg/L,P<0.05或0.01);肝组织脂联素蛋白表达在脂联素组强于对照组(5.39%±1.72%vs1.82%±0.36%,P<0.05),弱于ConA组(10.63±4.35%,P<0.05);肝组织脂联素mRNA表达在脂联素组和ConA组均强于对照组(0.46±0.17,0.51±0.21vs0.23±0.05,P<0.05或0.01);血清ALT水平及肝组织炎症程度脂联素组高于对照组(192.50±45.87U/Lvs44.71±21.29U/L;21.5±9.2vs8.4±4.3,均P<0.05),低于ConA组(616.00±171.50U/L,48.5±8.6,均P<0.05).结论:在ConA诱导的急性肝损伤中,肝组织脂联素蛋白及其mRNA的表达增强,但血清脂联素水平降低.脂联素对急性肝损伤小鼠起着抗炎作用.
AIM： To investigate the expression and role of adiponectin in murine acute liver injury. METHODS： Healthy male BALB/c mice were randomly divided into three groups： concanavalin A （ConA） treatment group （n = 8; intravenously injected with ConA at 30 mg/kg）, adiponectin treatment group （n = 5; intraperitoneally injected with adiponectin at 3 mg/kg at 12 and 3 hours before ConA injection） and normal control group （n = 8; intravenously injected with normal saline at 10 mL/kg）. Mice were sacrificed eight hours after ConA treatment to detect serum adiponectin and ALT levels, observe hepatic pathological changes and determine the expression of adiponectin mRNA and protein in the liver. RESULTS： Though no signif icant difference was observed in serum adiponectin levels between the adiponectin treatment group and the normal control group, serum adiponectin levels in these two groups were signif icantly higher than that in the ConA treatment group （15.4 ± 3.0 mg/L and 16.5 ± 2.8 mg/L vs 11.8 ± 2.1 mg/L, respectively; P〈0.05 or 0.01）. The expression intensity of adiponectin protein in the liver in the adiponectin treatment group was higher than that in the normal control group （5.39% ± 1.72% vs 1.82% ± 0.36%, P〈0.05） but lower than that in the ConA treatment group （10.63% ± 4.35%, P〈0.05）. The expression levels of adiponectin mRNA in the liver in the adiponectin treatment group and the ConA treatment group were signif icantly higher than that in the normal control group （0.46 ± 0.17 and 0.51 ± 0.21 vs 0.23 ± 0.05, respectively; P〈0.05 or P〈0.01）. The serum ALT level and the degree of hepatitis in the adiponectin treatment group were higher than those in the normal control group （192.50 ± 45.87 U/L vs 44.71 ± 21.29 U/L and 21.5 ± 9.2 vs 8.4 ± 4.3, respectively; both P〈0.05）, but lower than those in the ConA treatment group （616.00 ± 171.50 U/L and 48.5 ± 8.6, respectively; both P〈0.01）. CONCLUSION： The expression of adiponectin mRNA and protein in the liver is upregulated in ConA-mediated acute liver injury though serum adiponectin level is lowered. Adiponectin may have antiinB ammatory effects in ConA-induced acute liver injury.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第23期2346-2351,共6页
World Chinese Journal of Digestology
关键词
脂联素
刀豆蛋白A
急性肝损伤
Adiponectin Concanavalin A Acute liver injury
