摘要
作者曾用启动子探针型载体ｐＳＵＰＶ１，从环状芽孢杆菌Ｃ－２菌株（ＢａｃｉｌｕｓｃｉｒｃｕｌａｎｓＣ－２）中克隆到一个具有强启动功能的２．４ｋｂ片段ＢＣ６．对该片段所作的进一步缺失分析表明，其３′端约０．７ｋｂ片段具有单独的基因启动子功能．序列分析表明，ＢＣ６片段３′端有典型的原核生物的基因启动子结构．现将ＢＣ６的５′端的１．２ｋｂＸｂａⅠ片段重组于ｐＢＲ３２２的ＥｃｏＲＩ位点，观察其对两侧具有不同转录方向的Ａｍｐｒ与Ｔｃｒ基因表达的影响．结果表明，该片段能显著地增强或衰减与其紧密相连的Ｔｃｒ基因的表达，但对Ａｍｐｒ基因的表达却无明显的影响．
A 2.4kb fragment named BC6,containing promoter activity,had been cloned from Bacillus circulans C 2 using promoter probe plasmid vector pSUPV1.The result of further subcloning analysis indicated that the 3′ terminal 0.7kb region of the fragment showed its promoter activity.The 3′ terminal sequence analysis of BC6 demonstrated that the fragment had the typical prokaryotic promoter characters.The 5′ terminal 1.2kb Xba I fragment of BC6 was cloned into the Eco RI site of pBR322 to observe the effect of the inserted fragment on the expression of Amp r and Tc r gene,respectively.The result indicated that this Xba I fragment could increase or decrease the expression of Tc r gene,depending on the insertion direction,and have no obvious effect on the expression on Amp r gene.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
1998年第5期776-780,共5页
Journal of Sichuan University(Natural Science Edition)
基金
国家科技部"九五"攻关
关键词
环状芽孢杆菌
基因启动子
序列分析
DNA
Bacillus circulans ,gene promoter, sequence analysis,gene expression
