摘要
利用启动子探针型载体ｐＳＵＰＶ２首次从诸葛菜总ＤＮＡ中克隆了７个具有启动功能的ＤＮＡ片段。转化Ｅ．ｃｏｌｉ表明最高卡那霉素（ｋｎａ）抗性为１４０μｇ／ｍｌ，最低为２０μｇ／ｍｌ。含启动子片段的７个重组质粒分别命名为ｐＳＵＰＺ１－７。Ｓｏｕｔｈｒｅｎ印迹表明ｐＳＵＰＺ６对Ｋｎａ的抗性功能来源于诸葛菜的１０ｋｂ、５ｋｂ和３．９ｋｂ片段。
For the first time,seven DNA fragments having the ability of promotion were cloned from total DNA of O.violacecus with promoter probe vecter pSUPV2. The result of transforming E.coli showed that the highest kanamycin resistance level was 140μg/ml,and the lowest 20μg/ml.The seven recombinant plasmids with promoter fragments were named pSU P21-7.According to the result of Southern blot,the resistivity of pSUPZ6 was from the three DNA fragments of O.violaceccs with the MW.of 10kb,5kb and 3 9kb,respectively.
出处
《西南农业学报》
CSCD
北大核心
1997年第3期1-5,共5页
Southwest China Journal of Agricultural Sciences
基金
四川省科委资助
