摘要
土壤中的盐分是林木生长生存的限制因素。据M.Viant报道(1982),当土壤溶液中的NaCl浓度在0.05—0.1％时,杨树黑杨派中某些无性系尚可忍受,白杨派则可忍受0.05—0.3％的浓度。当NaCl浓度高于0.3％以上时,各种杨树均不能忍受。因此,培育耐盐碱杨树新品种是很有必要的。组织培养是一种诱导遗传变异的途径。P.J.Larkin认为培养细胞纯系变异。
China has large areas of Saline-alkah Soils, for which many breeding specialists including forest breeders and crop breeders have been being engaged in the breeding of saline tolerant plants. The experiment was conducted to induce the saline tolerant mutants in use of poplar callus line. In the experiment, MS was the basic culture base,2,4-D,NAA and BA were used as additives. Under a proper proportion, large numbers of embryoids or multiply shoots from some pieces of the leaves were induced. Later, these multiply shoots were induced in to the culture base with additive of NaCl. The increase gradient of NaCl. content was 1.5-3％. Totally, the subculture was continued for eight times. For each time, it was 30 days. Complete plants were formed from multiply shoots growing in the MS with 12.5％ NaCl content.
In the process of physiological inducement of saline tolerant mutants, explants demonstrated different degrees of tolerance as in the order of yellow leaf, indifferentiation of root organs, inhibition of root system development, normal growth of root system, and complete plant with good growth. The mutants not only increase the saline tolerance, but also produce an obvious variation of morphology and isoenzyme spectrum of peroxidase enzyme.
Different poplar species showed different reactionsin the inducement process. Saline tolerant (content 12.5％) mutants could be induced for Populus nigra, P. × beijingenses Cl '8000'. For P. diltoides Cl 'lux' and P. catheyana the root organs only could not differentiate. For P. maximowiczii, P. ×uramencana C1 'cima' and P. Popularis C1 '36' explants died as the NaCl content increased to 9％.
出处
《林业科学》
EI
CAS
CSCD
北大核心
1990年第4期359-362,共4页
Scientia Silvae Sinicae
关键词
杨树
耐盐
突变性
组织培养
研究
Poplar
Tissue culture
Mutant
Saline tolerant
