摘要
目的研究脑缺血大鼠纹状体多巴胺含量和Ｎａ＋，Ｋ＋－ＡＴＰ酶活性的变化以及ＮＭＤＡ受体拮抗剂氯胺酮对这些变化的影响。方法采用大鼠４－Ｖ－Ｏ造成急性脑缺血模型，缺血１０ｍｉｎ后分别应用ＨＰＬＣ－ＥＣＤ和比色法测量纹状体ＤＡ含量以及Ｎａ＋，Ｋ＋－ＡＴＰ酶活性。结果脑缺血前１５ｍｉｎ腹腔注射氯胺酮（２５ｍｇｋｇ－１和５０ｍｇｋｇ－１）能明显拮抗脑缺血时ＤＡ含量和Ｎａ＋，Ｋ＋－ＡＴＰ酶活性的降低。结论氯胺酮可通过阻断ＮＭＤＡ受体，抑制纹状体ＤＡ释放和Ｎａ＋，Ｋ＋－ＡＴＰ酶活性的降低，对抗脑缺血损伤。
AIM To study the effect of ketamine (KT) on the dopamine (DA) contents and Na+,K+ATPase activities in rats with transient cerebral ischemia. METHODS Animal model was prepeared by occlusion of four vessels (2 vertebral arteries and 2 common carotid arteries) (4VO). The DA contents were assayed by high performance liquid chromatography (HPLC) coupled with electrochemical detection (ECD), and Na+,K+ATPase activities were determined in rat striatum after 10 min of cerebral ischimia. RESULTS KT had cerebral protective effects so long as it was injected (25 mg·kg-1 and 50 mg·kg-1) intraperitoneally 15 min before ischemia began. KT obviously attenuated cerebral ischemiainduced reduction of DA contents and Na+,K+ATPase activities. CONCLUSIONS KT can block NMDA receptormediated increase of DA release and antagonize the inhibition of Na+,K+ATPase activities during cerebral ischemia, and this effects may be the important mechanisms by which ketamine protects cerebrum from ischemiainduced neuronic damage.
出处
《中国药理学通报》
CAS
CSCD
北大核心
1998年第1期47-49,共3页
Chinese Pharmacological Bulletin
基金
江苏省卫生厅科学基金
关键词
氯胺酮
脑缺血
纹状体
多巴胺
实验药理
ketamine
cerebral ischemia
striatum
dopamine
Na+,K+ATPase
