摘要
目的:检测原发性胃癌组织中18号染色体的杂合性丢失(lossofheterozygosity,LOH)情况.方法:联合应用激光捕获显微切割(lasercapturemicrodissection,LCM)-高可信度全基因组扩增(highfidelity-wholegenomeamplification,HF-WGA)-变性高效液相色谱(denaturedhighpressureliquidchromatography,DHPLC)方法,检测胃癌中18号染色体上短插入/缺失多态(shortinsertiondeletionpolymorphism,SIDP)标记的LOH.结果:在所检测的10例胃癌组织中3例呈现SIDP位点LOH(30%);9个SIDP位点中3个(MID148、MID150和MID352)发生LOH,其中MID150位点LOH见于3例胃癌组织.在1例胃癌组织中3个SIDP位点同时呈现LOH.结论:联合应用LCM和HF-WGA,经DHPLC分析SIDP标记,可进行肿瘤细胞中LOH检测.本研究为18号染色体上胃癌相关抑癌基因的研究提供一种新技术策略.
AIM:To detect loss of heterozygosity (LOH) on chromosome 18 in primary gastric carcinomas. METHODS:Short insertion deletion polymorphism (SIDP) markers on chromosome 18 were analyzed using high fidelity- whole genome amplification (HF-WGA) and denatured high pressure liquid chromotography (DHPLC) to detect LOH in gastric cancer cells purified by laser capture microdissection (LCM). RESULTS:LOH in 3 (MID148, MID150 and MID352) of 9 SIDP loci was found. Three of 10 samples of gastric cancer showed LOH at the same SIDP locus (MID150) (30 %) and one of them also demonstrated LOH at the other 2 SIDP loci (MID148, MID352). No LOH was detected in 6 of 9 SIDP loci. CONCLUSION: Detection of LOH in cancer cells can be achieved by the combination of LCM, HF-WGA and the analysis of SIDP marker by DHPLC. This study provides an alternative new way to map tumor suppressor genes on chromosome 18.
出处
《世界华人消化杂志》
CAS
2003年第3期310-313,共4页
World Chinese Journal of Digestology
基金
国家杰出青年科学基金资助课题
No.30125017~~
