摘要
为探讨甲醛对呼吸道的遗传性损伤和致肺癌机理，本研究以豚鼠的肺巨噬细胞为研究材料，采用碱洗脱膜过滤荧光分析方法，检测了甲醛对ＤＮＡ的损伤作用。结果表明，甲醛的急性细胞毒性较小，但能引起豚鼠肺巨噬细胞ＤＮＡ－蛋白质交链和ＤＮＡ单链断裂，并且交链和断裂的程度与甲醛浓度呈线性关系。说明甲醛对呼吸系统深部的细胞具有遗传性损伤，并进一步证实了甲醛具有遗传毒性。
In order to explore the hereditary damage on respiratory tract and the mechanism of lung cancer induced by formaldehyde(HCHO) Aldaline elution and fluorometric DNA assay was used to detected the DNA damage of alveolar macrophages in guinea pig caused by HCHO The results showed that the acute cytoxicity of HCHO was low But HCHO could induce DNA protein crosslinks (DPC) and DNA single strand breads (SSB) Moreover,both DPO and SSB had linear relations to HCHO concerntration So,HCHO could induce hereditary damage of cells in deep resplratory system These findings indicated once again that HCHO had genetic toxicity in character
出处
《环境与健康杂志》
CAS
CSSCI
CSCD
北大核心
1998年第6期253-255,共3页
Journal of Environment and Health
基金
北京市自然基金
关键词
甲醛
肺巨噬细胞
DNA
豚鼠
呼吸道损害
生物监测
Formaldehyde Alveolar macrophages DNA protoin crosslinks DNA single strand breaks
