摘要
目的：探讨进一步提高骨髓移植效果的新方法。方法：以昆明种小鼠急性放射病为模型，进行了骨髓程序移植后小鼠活存率及造血重建、急性移植物抗宿主病（ＧＶＨＤ）的观察。结果：骨髓程序移植可使小鼠移植细胞数降至１０５数量级，活存率达３０％；骨髓程序移植４×１０６个细胞时，于照射后１７天小鼠外周血白细胞、骨髓有核细胞计数显著回升，ＣＦＵＥ、ＣＦＵＧＭ、ＣＦＵＳ、ＣＦＵＦ已达正常，ＧＶＨＤ较一次移植１×１０７骨髓细胞组轻，活存率达６０％，显著高于一次骨髓移植组（３０％）。结论：骨髓程序移植可能充分利用了每次腾出的“龛位”，增加干细胞植入，却减少了移植细胞数，减轻了ＧＶＨＤ，提高了移植效果。
Objective:To enhance the grafting efficiency of bone marrow transplantation. Methods:Lethally irradiated recipient Kunming mice were transplanted with bone marrow cells from normal Kunming mice either in one large (10 7) number (BMT group) or in four separate small (10 5～10 6) numbers (scheduled BMT group ) and the survival rate, hematopoiesis reconstitution and acute graft versus host disease (aGVHD) were compared between the two groups. Results: Both BMT and SBMT (10 5×4) groups obtained the same survival rate of 30%. In SBMT (10 6×4) group, the peripheral WBC count, bone marrow nucleated cells and CFU E,CFU GM,CFU S and CFU F yields all returned to normal 17 days after irradiation. The degree of GVHD in SBMT group was less severe than that in BMT group, and the survival rate (60%) was significantly higher than that in BMT group (30%). Conclusion: SBMT can significantly enhance the grafting efficiency.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1998年第12期634-637,共4页
Chinese Journal of Hematology
基金
国家自然科学基金
关键词
骨髓移植
移植物抗宿主病
骨髓程序移植
Bone marrow transplantation Graft versus host disease Total body irradiation,mice
