摘要
利用SBR系统对聚磷菌进行了培养,并通过荧光原位杂交手段检测了系统中聚磷菌Candidatus Accumulibacter phosphatis的富集程度.聚磷菌也是一种普通异养菌,为了研究它的反硝化能力,排除了聚磷菌的正常释磷和吸磷过程,仅考察在不同碳源下反硝化性能.结果表明,乙酸和PHB都能成为聚磷菌反硝化的电子供体.当以乙酸为外在单一碳源时,其反硝化速率和PHB生成速率与起始硝酸盐浓度无关,但是当起始状态硝酸盐浓度越高时,消耗单位乙酸生成的PHB和硝酸盐还原量越小.以PHB为内在碳源和能源时,聚磷菌的反硝化速率呈现对于基质(硝酸盐)的零级动力学反应,比反硝化速率为0.973 3mg/(g.h),此外PHB平均比消耗速率为(以PHB计)2.462 6 mg/(g.h).
SBR reactor was performed to incubate polyphosphate accumulating organism （PAO）, and it was checked out of the system by fluorescence in situ hybridization. As PAO is a kind of ordinary heterotrophie bacteria, it was excluded the ability of phosphate release and uptake and it was considered only the capacity of denitrification of the target biomass. The results indicated that acetate and PHB can be the electron donors of PAO to denitrify. When fed with acetate, the denitrifying rate and PHB producing rate were independent of initial nitrate concentration. However, served as more nitrate in the reactor, it would be less PHB produced and fewer nitrate reducing when using same amount of acetate. In view of PHB stored as an internal carbon and energy source, it presented as a reaction of zero-order to the substrate by PAO to denitrify, such as nitrate, besides, the specific denitrifying rate was 0.973 3 mg/（g·h） and the specific PHB consuming rate was 2.462 6 mg/（g·h）.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2009年第10期2958-2962,共5页
Environmental Science
基金
国家自然科学基金项目(50808004
50628808)
"十一五"国家重大科技专项课题(2008ZX07317-007-105)
北京高校人才强教计划高层次人才资助项目(PHR20090502)
关键词
强化生物除磷
聚磷菌
反硝化
电子供体
enhanced biological phosphorus removal
polyphosphate accumulating organism（PAO）
denitrification
electron donor
