摘要
研究探讨人肝癌细胞分化过程中生化指标的变化。方法：用丁酸钠（ＳＢ）处理Ｂｅｌ－７４０２人肝癌细胞，测定甲胎蛋白（ＡＦＰ）、γ－谷氨酰转肽酶（γ－ＧＴ）、醛缩酶（ＡＬＤ）、酪氨酸－α－酮戊二酸转氨酶（ＴＡＴ）、碱性磷酸酶（ＡＬＰ）的比活性变化，并观察细胞增殖速度改变。结果：２．５ｍｍｏｌ／Ｌ和５ｍｍｏｌ／ＬＳＢ处理后，Ｂｅｌ－７４０２细胞增殖明显抑制，ＡＦＰ分泌量明显低于对照组细胞（Ｐ＜０．０５或０．０１），反映肝细胞癌变标志酶γ－ＧＴ和ＡＬＤ比活力也明显降低（Ｐ＜０．０１或０．０５），而反映肝细胞分化的酶ＴＡＴ和ＡＬＰ比活力则明显升高（Ｐ＜０．０５或０．０１）。结论：ＳＢ使人肝癌细胞分化过程中ＡＦＰ、γ－ＧＴ、ＡＬＤ、ＴＡＴ、ＡＬＰ均有明显变化，可用这些生化指标来观察肝细胞恶性表型的逆转。
Purpose:This study was to find the changes of biochemical index during differentiating of the human hepatocarcinoma cells.Method:The Bel 7402 human hepatocarcinoma cells were treated by sodium butyrate(SB),and the specific activities of γ glutamy transpeptidase(γ GT),aldolase(ALD),tyrosine α ketoglutarate aminotransferase(TAT),alkeline phosphatase(ALP)and the secreting quantities of α fetoprotein(AFP)were determined and the proliferation rate of the cells was observed.Results:After treating with 2.5mmol/L and 5mmol/L SB,the proliferation rate of the Bel 7402 cells was significantly decreased,the AFP secreting amounts was significantly lower than the control cells ( P <0.05 or 0.05),and the specific activities of γ GT and ALD which marked the canceration of the liver cells were significantly decreased ( P <0.01 or 0.05),but the specific activities of TAT and ALP which reflected the differentiation of the liver cells were significantly increased( P <0.05 or 0.01).Conclusions:SB could make the AFP,γ GT,ALD,TAT and ALP change significantly during differentiating of the human hepatocarcinoma cells ,and these biochemical indexes could be used to observe the reversing of hepatocarcinoma malignant phenotype.
出处
《赣南医学院学报》
1998年第3期193-197,共5页
JOURNAL OF GANNAN MEDICAL UNIVERSITY
关键词
丁酸钠
人肝癌细胞
分化
甲胎蛋白
酶学指标
Sodium butyrate,Human hepatocarcinoma cell,Differentiation,α fetoprotein,Enzyme
