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利用噬菌体展示技术制备识别酵母RNR3原核表达片段的单链抗体

Preparation scFvs against the Procaryotic Truncated Fragment of RNR3 in an Unimmuned Antibody Phage Display Library
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摘要 选取酵母细胞RNR3蛋白序列中高保守片段:145-298位氨基酸序列,命名为RNR3h进行原核表达.并利用噬菌体展示技术从非免疫小鼠噬菌体展示抗体库中淘选到能与该原核表达产物特异性结合的单链抗体.得到的3种单链抗体均识别原核表达片段的空间抗原位点.为寻找酵母RNR1和RNR3的诱导表达水平与DNA损伤信号的相互关系,将真核生物RNRs发展为检测环境化学致癌物DNA损伤效应的生物标志物进行了尝试. In this study, a selected homologous peptide from Saccharomyces cerevisiae RNR3 （loc145- loc298） named RNR3h was expressed in E. coli, and the purified protein is used as an antigen to prepare specifically binding scFvs （single chain Fragment-variable） from an unimmuned mouse sourced antibody phage display library. Three different scFvs （single chain Fragment-variable region） that recognize the spatial epitope of RNR3h were obtained and further analyzed. Preliminary studies were made to discover the RNR1 and RNR3＇s expression features that induced by DNA damage, and to develop RNR3 as a biomarker to detect the chemical carcinogens in environment by reacting to DNA damage.
作者 李佳 宋波 王玉珍 饶瑜 戴和平
机构地区 中国科学院水生生物研究所 中国科学院研究生院
出处 《武汉大学学报（理学版）》 CAS CSCD 北大核心 2009年第5期563-568,共6页 Journal of Wuhan University:Natural Science Edition
基金 国家自然科学基金资助项目(30640055 20777091)
关键词 核糖核苷酸还原酶 酵母RNR3 噬菌体展示技术 单链抗体 RNR RNR3 phage display technology single chain Fragment-variable（scFvs）
分类号 Q7 [生物学—分子生物学]
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参考文献20

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二级参考文献3

  • 1Yang F, He J, Lin X, et al. The complete genome sequence of the shrimp White Spot Bacilliform Virus [ J]. J. Virol. 2001,75:11811-11820.
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武汉大学学报（理学版）
2009年 第5期

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