摘要
目的：用固相萃取一毛细管气相色谱分析方法测定药材中的六六六、滴滴涕残留量。方法：样品以混合溶剂超声提取、Florisil固相萃取小柱净化处理后,采用DB-5毛细管气相色谱柱分离样品,电子捕获检测器检测。结果：六六六、滴滴涕在0.005-0.100μg/ml范围内有良好的线性关系,相关系数均大于0.999。平均回收率为87.5%-99.0%,RSD为1.13%~2.67%。最小检测量为0.001-0.008 mg/kg。结论：本法简便、灵敏、准确、重现性好,可用于药材中六六六、滴滴涕残留量测定。
Objective：To establish solid - phase extraction and capillary gas chromatography for the determination of HCH, DDT in medicinal plant. Methods：The HCH, DDT pesticide residues were extracted from samples with solvents by ultrasonic and cleaned up by Florisil solidphase extraction column. Then, the extract was separated by capillary column and detected by electron capture detector. Results：There was good 1 iner relationover the range of 0. 005 -0. 100 μg/ml. The average recoveries and RSD were in the range of 87.5% - 99. 0% and 1.13% N 2.67% at three spiked mixed organOchl orine pesticides levels, respectively. The limits of detection were 0.001 - 0. 008 mg/kg. Conclusion： This method is effective, fast, accurate, repeatable and suitable for analysis of HCH and DDT residues in traditional medicines materials.
出处
《中国卫生检验杂志》
CAS
2009年第10期2270-2271,共2页
Chinese Journal of Health Laboratory Technology
关键词
药材
六六六、滴滴涕
固相萃取
气相色谱
Medicinal plant
HCH
DDT
Solid-phase extraction
Capillary gas chromatography
