摘要
为了探讨运动性疲劳过程中理气扶正中药作用的分子机理以及寻求安全有效的消除运动性疲劳的中药复方制剂，设计了强化训练导致疲劳的大鼠模型，用定量反转录聚合酶链式反应（ＱｕａｎｔｉｔａｔｉｖｅＲＴＰＣＲ）方法，研究了疲劳恢复过程中，大鼠骨骼肌α－肌动蛋白（ａ－ａｃｔｉｎ）基因表达的变化。结果表明：所有运动施药组的表达均高于安静对照组，且强化训练恢复２４小时组（Ｅ－２４）、强化训练恢复４８小时组（Ｅ－４８）显著高于安静对照组。强化训练施药组较相应的强化训练非施药组均无明显差异。结果提示理气扶正中药在大鼠运动性疲劳恢复过程中对骨髓肌α－肌动蛋白基因表达的作用具有时效性。
In order to investigate the molecular mechanism of Li-qi Fu-zhen Chinese medicine during removing exer- cise-induced muscle fatigue and seek for an effective Chinese medicine compound dealing with exercise fatigue , an SD rat model of intensive training was constructed and the expression of α-actin of skeletal muscle during re- freshine was studied by the quantiative RT-PCR( QRT-PCR) . It shows that the expression level of all groups treated with the Chinese medicine is higher than that of rest control,and the expression after and 48 hours from intensive training increase significantly than that from rest control.There is no-significant difference be- tween the groups treated and non-treated with the Chinese medicine.These results suggest that a timing exists while Li-qi Fu-zhen Chinese medicine functions on expression of α-actin in skeletal muscle .
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
1998年第4期309-311,共3页
Chinese Journal of Sports Medicine
基金
国家自然科学基金
关键词
理气扶正中药
疲劳
骨骼肌
Α-肌动蛋白
表达
Li-qi Fu-zhen Chinese medicine
fatigue
skeletal muscle
a-actin
expression
