摘要
目的:研究江苏地产白首乌总苷对四氯化碳所致大鼠肝纤维化的保护作用。方法:Wistar大鼠随机分6组,正常组、模型组给1%CMC-Na,阳性药组给氢化可的松3.5 mg.kg-1,白首乌低、中、高剂量组分别给予白首乌总苷4,8,16 mg.kg-1。大鼠每周2次灌胃50%CCl4花生油2 mL.kg-1,连续8周。造模与给药同时进行。8周后取血,计算大鼠的肝脾指数,检测大鼠血清中谷丙转氨酶(GPT),谷草转氨酶(GOT)的活力;检测血清中透明质酸(HA)、Ⅲ型前胶原(PCⅢ)的含量;检测肝脏组织中超氧化物歧化酶(SOD)活性、羟脯氨酸(HyP)和丙二醛(MDA)含量。观察大鼠肝组织病理变化。结果:江苏地产白首乌总苷能明显降低CCl4所致肝纤维化大鼠的肝脾指数(P<0.05)和血清中GPT,GOT活性(P<0.01),降低血清中HA,PCⅢ的含量(P<0.01);降低肝组织中MDA的水平和HyP的含量(P<0.01),亦能升高肝组织中SOD的活性(P<0.01)。结论:江苏地产白首乌总苷具有显著的抗肝纤维化的作用。
Objective ： To investigate the effect of general glycosides from Cynanchun auriculatum of Jiangsu on liver fibrosis of rats. Method： Seventy-two Wistar rats were randomly divided into normal group, model group, BSW three doses treated group and hydrocortisone treated group. CC14 （50% , 2 mL ·k^-1 ） was orally administraeated twice a week for 8 weeks. The liver and spleen indices were observed. The level of serum GPT, GOT, and HA, PCⅢ and the level of SOD, HyP, MDA in liver homogenates was also measured. The histopathologic change in hepatic of rats was examined. Result： The elevation of serum GPT, GOT, HA, PC Ⅲ, MDA and HyP and the content of liver homogenates were attanuated remarkably by BSW treatment. BSW groups also increased the level of SOD of liver homogenates, and make the fibrotic liver better. Conclusion： The general glycosides in C. auriculatum of jiangsu province have an anti-hepatic fibrosis ettect on CC4-induced fibrosis rats, the mechanisms might be associated with its anti-oxidative action.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第19期2508-2511,共4页
China Journal of Chinese Materia Medica
基金
江苏省科技招标项目(S200406005)
关键词
肝纤维化
白首乌
脂质过氧化
liver fibrosis
Cynanchun auriculatum
lipid peroxidation
