摘要
[目的]研究不同贮藏方式对4种桉树花粉活力的影响,探索出适宜桉树花粉贮藏的低温条件和贮藏方式。[方法]以尾叶桉、尾叶桉×巨桉、巨桉和邓恩桉花粉为试验材料,对其进行常温、低温、低温冷冻和超低温冷冻贮藏,并对不同贮藏时间内的花粉活力进行检测。[结果]适合桉树花粉萌发的最佳培养基为20%蔗糖+6%琼脂+0.1‰硼酸;贮藏方式和贮藏时间对花粉活力有显著影响,常温真空贮藏,尾叶桉和巨桉花粉贮藏30d后,萌发率降至10%以下,尾叶桉×巨桉和邓恩桉花粉贮藏50d后,花粉萌发率降至10%以下,低温冷冻和超低温冷冻贮藏1年的桉树花粉萌发率仍保持在35%以上;不同的解冻方式对花粉萌发率有显著影响,以38℃水浴3min化冻效果最好。[结论]实际工作中选用低温冷冻贮藏花粉,可以得到既经济又高效的收益。
[ Objective ] The aim was to research the effects of different storage methods on pollen viability of 4 eucalyptus, and explore the suitable low temperature condition and storage method tor pollen storage of eucalyptus. [ Method ] Taking the pollens of E. urophylla S. T. Blake, E. urophyUa S.T. Blake × E. grandis W. Hill exmaiden, E. grandis W. Hill exmaiden and E. dunnii Maiden as test material, the pollens were preserved in room temperature, low temperature, low temperature freezing and ultra low temperature freezing. The pollen viabihties during different storage time were determined. [ Result] The best medium for eucalyptus pollen germination was 20% sucrose ＋ 6% agar ＋ 0.1%v boric acid. Storage methods and time had significant effect on pollen viability. After 30 days, the pollen germination rate of E. urophylla and E. dunnii declined to 10%, after 50 days, the pollen germination rate of E. urophylla S.T. Blake× E. grandis W. Hill exmaiden and E. dunnii Maiden declined to 10% in room temperature. The pollen was preserved in low temperature freezing and ultra low temperature freezing for one years, the pollen germination rate was still more than 35%. Pollen germination rate was also affected by different thawing methods, and 38 ℃ water bath for 3 minutes was the best method. [ Conclusion ] Selecting low temperature freezing method to preserve pollen in practical work, it can obtain economic and efficient benefit.
出处
《安徽农业科学》
CAS
北大核心
2009年第30期14997-15000,共4页
Journal of Anhui Agricultural Sciences
基金
国家林业局"948"项目(2005-4-57)"桉树人工制种及强化育种极少引进"
国家十一五科技支持专题"高产优质桉树速生材树种新品种选育"(2006BAD01A15-4)
"桉树速生丰产林长期生产力维持与持续经营技术研究"(2006BAD24B0203)项目
关键词
桉树
贮藏方式
花粉活力
影响
Eucalyptus
Storage method
Pollen vitality
Effect
