摘要
利用化学发光法研究锦橙皮中多甲氧基黄酮提取物(PMFs)的体外抗氧化活性及其对DNA损伤的保护作用。分别采用Pyrogallol-Luminol化学发光体系、CuSO4-Phen-VC-H2O2化学发光体系和H2O2-Luminol化学发光体系测定PMFs对超氧阴离子自由基、羟基自由基及过氧化氢的清除能力。采用CuSO4-Phen-VC-H2O2-DNA化学发光体系衡量PMFs对DNA损伤的保护作用。结果表明:PMFs可有效清除超氧阴离子自由基、羟基自由基和过氧化氢的半数抑制率(IC50)分别为6.1、7.8、0.6μg/ml;PMFs对DNA损伤能产生较好的保护作用,其保护机制属于抑制型,IC50为4.0μg/ml。
The antioxidant activity of polymethoxylated flavones （PMF） extracts from peels of C. sinensis was evaluated by chemiluminescence （CL） method in vitro, the scavenging capabilities of PMFs against superoxide anion, hydroxyl free radial, and hydrogen peroxide were determined by Pyrogallol-Luminol system, CuSO4-Phen-VC-H2O2 system, and Luminol-H2O2 system, respectively, and the prevention effect of PMFs on DNA damage was evaluated by CuSO4-Phen-VC-H2O2-DNA CL system. Results showed that PMFs exhibited effective antioxidant activity to scavenge superoxide anion, hydroxyl free radial, hydrogen peroxide, and prevention on DNA damage in a dose-dependent manner. The IC50s of PMFs to superoxide anion, hydroxyl free radial, and hydrogen peroxide were 6.1, 7.8, 0.6 and 4.0μg/ml, respectively.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第19期19-22,共4页
Food Science
基金
农业部"948"项目(2006-Z-25)
关键词
柑橘
多甲氧基黄酮(PMFs)
抗氧化
化学发光
citrus
polymethoxylated flavones （PMFs）
antioxidant activity
chemiluminescence
