摘要
目的:研究桔梗与氯丙嗪联合应用对大鼠脑纹状体单胺类神经递质多巴胺的影响,探讨中西药配伍应用后可能发生的药物相互作用与机制。方法:大鼠随机分成4组:生理盐水组、桔梗(生药15.2 g.kg-1.d-1)单独给药组、氯丙嗪(20mg.kg-1.d-1)单独给药组、氯丙嗪与桔梗(20 mg.kg-1.d-1+15.2 g.kg-1.d-1)合并给药组,每组6只,给药10 d后,利用微透析法在大鼠脑纹状体取样,HPLC检测透析液中多巴胺水平。结果:药后140 m in(取样点80 m in)时,氯丙嗪+桔梗组中多巴胺水平(1.52±0.34)μg.L-1显著高于氯丙嗪组(1.25±0.22)μg.L-1(P<0.05)和生理盐水组(1.06±0.24)μg.L-1(P<0.01)。结论:桔梗与氯丙嗪合用后大鼠脑内多巴胺水平升高,治疗时同时给予桔梗,可以减少氯丙嗪的用量,达到保持疗效,降低其不良反应的目的。
Objective ： To study the effects of chlorpromazine combined with Platycodon grandiflorum on the striatal extracellular dopamine level in rats and to research the interaction and the mechanism of action after combining traditional Chinese medicine with western medicine. Method： Twenty four rats were randomly assigned into four groups： the control group, Platycodon group, chlorpromazine group and chlorpromazined combined with P. grandiflorum group. The level of dopamine in CSF microdialysis samples was detected with high performance liquid chromatography with electrochemical detector after administration for 10 days. Result： The CSF level of DA （ 1.52 ±0. 34） μg · L^-1 was significantly higher （P 〈0.01 ） in chlorpromazine combined with P. grandiflorum group than that in the chlorpromazine group （ 1.25 ±0. 22） μg · L^-1 （ p 〈 0. 05 ） and that in the normal control （ 1.06 ±0. 24 ）μg · L^-1 （ P 〈 0. 01 ）. Conclusion： The combining utilization of P. grandiflorum and chlorpromazine may increase the DA concentration of monoamine neurotransmitters, which results in under the therapeutic effect is maintained, the dosage of chlorpromazine used to individuals are decreased and the incidence rate of the adverse reactions of chlorpromazine will descend.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第18期2386-2389,共4页
China Journal of Chinese Materia Medica
关键词
氯丙嗪
桔梗
微透析
多巴胺
电化学检测
chlorpromazine
Platycodon grandiflorum
microdialysis
dopamine
electrochemical detection
