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DLK1在急性白血病中的表达及其在K562细胞红系分化中的作用 被引量：2

Expression of DLK1 gene in acute leukemias and its function in erythroid differentiation of K562 cell line
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摘要 目的:探讨急性白血病(acute leukemia,AL)患者骨髓单个核细胞DLK1基因的表达水平及其在K562细胞红系分化中的作用。方法:采用RT-PCR对65例AL患者及34例正常骨髓对照进行DLK1mRNA水平的检测,对其中20例AL患者及13例正常骨髓用Western印迹法检测DLK1蛋白表达水平。培养K562细胞,用氯化高铁血红素(hemin)诱导其分化,观察DLK1在红系分化中的变化。结果:在AL患者和正常骨髓对照的骨髓单个核细胞中DLK1基因均存在明确表达。DLK1mRNA的表达水平AL组高于对照组,差异有统计学意义(P=0.018),但在ALL和ANLL之间DLK1mRNA的表达差异无统计学意义(P>0.05),且DLK1mRNA的表达水平与患者初诊时骨髓原始细胞数、外周血白细胞数及血小板数无关。对部分样品检测DLK1蛋白的表达,发现AL患者DLK1蛋白阳性表达率高于对照组,与mRNA表达趋势一致。在hemin诱导K562细胞向红系分化过程中,DLK1mRNA的表达水平逐渐下降。结论:DLK1基因可能参与了AL的发病过程,但DLK1mRNA的表达水平与AL患者某些临床特征无相关性。DLK1基因可能抑制K562细胞向红系分化。 Objective To determine the expression of DLK1 gene in acute leukemias （AL） and its function in erythroid differentiation of K562 cells. Methods We detected the expression of DLK1 gene in 65 different acute leukemia categories （a test group ） and 34 normal bone marrow controis （a control group） with RT-PCR. DLK1 protein in 20 out of the 65 AL patients and 13 of the 34 controls was assayed by Western blot. The K562 cell line was induced to erythroid differentiation by hemin. We observed the relationship between its expression and erythroid differentiation. Results Both leukemia cells and normal marrow cells expressed DLK1. The expression of DLK1 mRNA in patients in the test group was higher than that in the control group （ P = 0.018 ） , while there was no significance between acute lymphoblastic leukemia and acute myelogenous leukemia （P 〉 0.05 ）.The expression of DLK1 mRNA in the test group at onset had no relation with the WBC and platelet count in the total peripheral blood, and the same was true for blast cell rates in bone marrow cells. The level of DLK 1 protein in the test group was higher than that in the control group, which was consistent with the mRNA expression （ P = 0. 042 ）. The expression of DLK1 mRNA decreased gradually with K562 cells towards hemin-induced erythroid differentiation. Conclusion DLK1 gene may be involved in leukemia, but the mRNA level of DLK1 has no relation with some clinical characteristics of AL patients at onset. DLK1 may inhibit the erythroid differentiation of K562 cells.
作者 唐雪元 龙潺 王成红 肖广芬
机构地区 中南大学湘雅三医院血液内科 深圳市第三人民医院
出处 《中南大学学报（医学版）》 CAS CSCD 北大核心 2009年第9期886-891,共6页 Journal of Central South University ：Medical Science
基金 湖南省自然科学基金(09JJ3067)~~
关键词 急性白血病 DLK1 基因表达 红系分化 acute leukemia DLK 1 gene expression erythroid differentiation
分类号 R733.71 [医药卫生—肿瘤]
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参考文献11

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同被引文献37

  • 1陈地龙,刘永刚,王亚平,陈婷梅,郑敏,姜蓉.人参多糖对K562细胞增殖抑制及诱导分化的实验研究[J].第三军医大学学报,2005,27(6):517-520. 被引量：25
  • 2陈地龙,李静,刘永刚,王亚平,陈婷梅,郑敏,姜蓉.人参多糖对K562细胞人粒-巨噬细胞集落刺激因子和促红细胞生成素及其受体表达的影响[J].中草药,2006,37(10):1526-1529. 被引量：2
  • 3于峰,李锦军.DLK1在干细胞和肿瘤中的作用[J].生命的化学,2006,26(6):529-532. 被引量：6
  • 4Laborda J,Sausville EA,Hoffman T,et al.DLk,a putative mammalian homeotic gene differentially expressed in small cell lung carcinoma and neuroendocrine tumor cell line[J].J Biol Chem,1993,268(6):3817-3820.
  • 5Moore KA,Pytowski B,Witte L,et al.Hematopoietic activity of a stromal cell transmembrane protein containing epidermal growth factor-like repeat motifs[J].Proc Natl Acad Sci USA,1997,94(8):4011-4016.
  • 6Ditte CA,Stine JP,Louise HJ,et al.Characterization of dlk1^+ cells emerging during skeletal muscle remodeling in response to myositis,myopathies and acute injury[J].Stem Cells Express,2009,8(1):12-19.
  • 7Samulin J,Berg PR,Sundvold H,et al.Expression of DLK1 splice variants during porcine adipocyte development in vitro and in vivo[J].Animal Genetics,2008,40(7):239-241.
  • 8Moon YS,Smas CM,Lee K,et al.Mice lacking paternally expressed Pre-1/Dlk1 display growth retardation and accelerated adiposity[J].Mol Cell Biol,2002,22(15):5585-5592.
  • 9Smas CM,Sul HS.Pre-1,a protein containing EGF-like repeats,inhibits adipocyte differentiation[J].Cell,1993,73(8):725-734.
  • 10Liang Li,Stephen JF,Ravi B.Expression of DLK1 in hematopoietic cells results in inhibition of differentiation and proliferation[J].Oncogene,2005,24(10):4472-4476.

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中南大学学报（医学版）
2009年 第9期

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