摘要
为了建立一种简便、快速检测新城疫病毒(Newcastle disease virus,NDV)的方法,本试验采用柠檬酸三钠还原法制备胶体金颗粒,标记NDV单克隆抗体6C4制备免疫检测探针。在硝酸纤维素膜上,用微定量喷头喷好1条病毒检测线(T线)和1条羊抗鼠抗体质控线(C线),制备免疫层析检测试纸条,使用该制备好的检测试纸条可在10 min内检测出新城疫病毒。试纸条检测NDV的灵敏度比HA试验结果提高8倍,与禽流感病毒未出现交叉反应,用缓冲液对照测试,结果为阴性,在密封、干燥、低温的条件下,试纸条的灵敏性与特异性没有明显变化。本研究建立的NDV免疫层析检测法具有特异、灵敏、稳定、操作简单等特点,符合现场快速检测的要求。
To develop a simple, rapid and high efficiency immunochromatographic assay for Newcastle disease virus （NDV）. The sodium citrate reduction was used to make colloidal gold particles which will conjugate with NDV monoclonal antibody as immunoassay probes. The test line was sprayed on the nitrocellulose membrane, while a sheep anti-mouse antibody quality control line （C line） was sprayed. The strip could detect NDV in 10 min. The detection of NDV was more sensitive than the HA test results by improving 8 times. NDV had no cross-reactions with AIV. With buffer as control testing, the result was negative. Storage of the strips in a sealed, dry, low temperature conditions had not changed their sensitivity and specificity. The research revealed that the strip for detection of NDV has high specificity, sensitivity and stability. Its rapid detection and easy operation make it suitable for widely use.
出处
《中国畜牧兽医》
CAS
北大核心
2009年第9期122-125,共4页
China Animal Husbandry & Veterinary Medicine
基金
"十一五"国家科技支撑计划项目(2006BAK10B06
2006BAK10B09)
质检公益性行业科研专项项目(10-47)
关键词
新城疫病毒
金标试纸条
胶体金
Newcastle disease virus
immunochromatographic strip
colloidal gold
