摘要
本试验通过玻璃化冷冻前不同浓度细胞松弛素B(cytochalasin-B,CB)预处理来分析CB对牛GV期卵母细胞固体表面玻璃化(SSV)冷冻后发育潜力的影响。试验结果表明,玻璃化冷冻后,CB处理组和未处理组之间卵母细胞成熟率差异不显著(P>0.05),但均显著低于体外培养组。说明试验中所用CB浓度(2.5、7.5、15、20和30μg/mL)对牛GV期卵母细胞玻璃化冷冻效果的改善作用不明显。
In the present study, effects of concentration of cytochalasin B（CB） on solid surface vitrification （SSV） of bovine GV-stage oocytes were examined. The results demonstrated that no significant differences were observed in vitro maturation rate among with or without CB pre-treatment,while they were all significantly lower than that in the IVM group. In conclusion, different concentration CB（2. 5,7. 5,15,20 and 30 μg/mL）treatment before vitrification did not improve the IVM rate following the vitrification of immature bovine oocytes.
出处
《中国畜牧兽医》
CAS
北大核心
2009年第9期101-104,共4页
China Animal Husbandry & Veterinary Medicine
基金
新疆高技术研究与发展项目(200841122)
基金项目(200821182)
国家863项目(2008AA101007)
