摘要
目的探讨玻璃化冷冻对小鼠卵母细胞孤雌发育的影响。方法以小鼠MⅡ期卵母细胞为模型,将未经冷冻直接进行培养的卵母细胞设为对照组,将经玻璃化冻融后再进行培养的卵母细胞设为试验组,观察是否出现单原核或卵裂及囊胚形成情况。结果未经玻璃化冻融处理的卵母细胞有2.96%发生孤雌发育,而经玻璃化冻融处理的卵母细胞孤雌发育率达13.50%,两者存在明显差异(P<0.05),但均未见囊胚形成。结论玻璃化冷冻可诱发卵母细胞发生孤雌发育,但这些孤雌发育的胚胎难以发育到囊胚阶段。
Objective ： Effect of vitrificaton on the parthenogenetical development of mouse mature occyte were investigated. Methods： In the control group, no -vitrified ooeytes were cutured dereetly; In the experimental group, the vitrified oocyte were cultured to explore whether vitrification caused parthenogenetical development or not. Results： The parthenogenetieal development of experimental group was significantly higher than that of the control group （13, 50% vs 2.96% , P 〈0.05） , but no blastoeyst developed in these two arouos. Conclusion. Vitrification might induce parthenogenetical development of mouse mature oocyte.
出处
《中国优生与遗传杂志》
2008年第11期98-99,共2页
Chinese Journal of Birth Health & Heredity
基金
广西自然科学基金资助项目(桂科自0542058)
广西卫生厅科研基金资助项目(Z2004024
Z2007013
Z2007012)
关键词
卵母细胞
玻璃化冷冻
小鼠
孤雌发育
Oocytes
Vitrification
Mouse
Parthenogenetical development
