摘要
应用衍生于水稻剩余杂合体的分离群体,开展株高QTL的检测和精细定位。应用1个在水稻第6染色体短臂约7.3 Mb区间分离、背景基本纯合的F2:3群体,种植于海南、浙江两地,检测到2个控制株高的QTL;然后,针对两地间作用稳定的qPH6-1,筛选出3个杂合区间缩小且呈阶梯状排列的单株,衍生F2群体,进一步验证了qPH6-1的作用,并将其界定于距离为96.4 kb的SSR标记RM3414和RM19417之间;最后,应用分离区间进一步缩小且呈阶梯状排列的3个F2群体,将qPH6-1定位于距离为51.7 kb的STS标记Si2925和SSR标记RM19417之间。基因组位置比较结果显示,该基因与所有已定位或克隆的水稻矮秆、半矮秆基因均非等位。
Populations derived from residual heterozygous lines （RHLs） were used for the detection and fine mapping of quantitative trait loci （QTLs） for plant height in rice. An F2：3 population which segregates in a 7.3 Mb region on the short arm of rice chromosome 6 in an isogenie background were transplanted in two trial sites in Hainan and Zhejiang Provinces, respectively. Two QTI.s for plant height were detected, of which qPH6-1 exhibited a consistent effect across the two sites. Three plants having smaller heterozygous segments which overlap the qPH6-1 region were selected. Using the three F2 populations produced, qPH6-1 was validated and delimitated to a 96.4-kb region flanked by SSR markers RM3414 and RM19417. Three more F2 populations which segregate in smaller regions covering qPH6-1 were developed. The qPH6-1 locus was delimited to a 51.7-kb region flanked by STS marker Si2925 and SSR marker RM19417. It was also shown that this gene is nonallelic to all the major genes for dwarfism mapped or cloned in rice.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2009年第5期470-474,共5页
Chinese Journal of Rice Science
基金
浙江省自然科学基金资助项目(R306285)
农业部中国超级稻研究与示范项目(2009-6)
中央级公益性科研院所基本科研业务费专项资助项目
关键词
株高
数量性状基因座
精细定位
剩余杂合体
水稻
plant height
quantitative trait locus
fine mapping
residual heterozygous line
rice
