摘要
为了建立重庆板角山羊精液的细管冷冻保存方法,实验进行了不同冷冻稀释液(配方Ⅰ、Ⅱ、Ⅲ)、不同冷冻保存剂(甘油、EG)及不同离心速度(1000、1200、1400r/min)对重庆板角山羊细管精液冷冻保存效果的研究,结果表明:配方Ⅱ对重庆板角山羊精液的冻后活率显著优于配方Ⅰ和Ⅲ(P<0.05)。在配方Ⅱ中添加相同剂量(5%)的EG和甘油,精液冻后活率差异不显著(P>0.05)。以1200r/min的速度对山羊鲜精作离心处理后,冻后活率相对于对照组有所提高,但差异不显著(P>0.05)。
To establish the cryopreservation method of Chongqing Banjiao Goat semen by straws, different extender （extender Ⅰ, Ⅱ, Ⅲ）, different cryoprotectant（Glycerol and EG） and different centrifugal speed（1 000, 1 200 r/min and 1 400 r/min） were studied. The results showed that：the extender Ⅱ was the best among the extenders and its sperm motility after freezing and thawing was significantly higher than that of other two extenders （P 〈 0.05）; When added the same concentration （5%） of EG and glycerol into the extender Ⅱ, sperm quality showed no significant difference （P 〉 0.05）;Centrifuging semen at 1,200 to remove the seminal plasma before cryopreservation could slightly improve the frozen sperm motility.
出处
《中国畜牧杂志》
CAS
北大核心
2009年第17期16-19,共4页
Chinese Journal of Animal Science
基金
国家科技支撑计划项目(2007BAD51B01)
重庆市科技攻关计划项目(CST2006AA1008)
关键词
细管冷冻精液
EG
甘油
离心
重庆板角山羊
Chongqing Banjiao Goat
the straw frozen semen
glycerol
EG
centrifugal speed
