摘要
云南以大理州冻精站原来使用的波尔山羊细管冻精稀释液作为对照配方,设计A配方和B配方进行改进,结果表明A配方保存的冻后精子活力、顶体完整率、冻后有效精子数等主要性状显著高于原配方和B配方(P<0.01);利用含有卵黄的稀释液进行波尔山羊细管冻精的稀释,能够明显提高波尔山羊精子的冻后活力、顶体完整率、冻后有效精子数等(P<0.05)。原配方中添加维生素C,可有效提高精子的冻后活力(P<0.05);添加维生素B_(12)是添加筛选的三种维生素中最能显著提高波尔山羊细管冻精保存效果(P<0.05);使用乙二醇具有更好的抗冻作用,大大提高了冻后精子复苏率,明显改善了细管冻精的保存效果(P<0.05),可作为甘油的替代品。在1～7%十个浓度梯度中,以3%与4.5%的甘油浓度状态下的精子冻后活力、顶体完整率、总存活时间、精子畸形率、冻后有效精子数等性状的性能表现最佳,是波尔山羊细管冻精最佳的甘油浓度;尽管使用7%甘油浓度的精液性状接近于3%和4.5%的,但这里不建议使用较高的甘油浓度。
Treatment A and B were taken out to improve the former diluents of straw frozen semen on Boer goat at Frozen Semen Station of Dali Prefecture, Yunnan. The results showed that the performances of Treatment A on sperm progressive motility after thawing, acrosome intersity after thawing, and effective spermatozoa count after thawing were higher than those of Treatment B and the former diluents, respectively （P〈0.01）. The performances of frozen semen after thawing could be improved after adding egg yolk to the diluents, of which the progressive motility after thawing, acrosome integrity after thawing, and effective spermatozoa count after thawing increased （P〈0.05）. The motility of frozen semen after thawing could be improved after adding vitamin C to the diluents （P〈0.05）, and it was optimal that vitamin B12 were used to diluents prior to Vitamin C and E （P〈0.05）. Glycol was the substitution of glycerol as better cryoprotective preservation, which improved the sperm resuscitation rate after thawing and eryoprotective effects in the process of straw frozen semen productions （P〈0.05）. Of the ten gradient glycerol densities （1~7%）, the optimal glycerol concentrations were 3% and 4.5%, of which the progressive motility of frozen semen after thawing, aerosome integrity after thawing, total sperm survival time after thawing, effective spermatozoa count were generally first-grade. Although the corresponding semen qualities at the level of 7% glycerol were perfect close to those of 3% and 4.5%, herein it was not recommended to use in straw frozen semen due to its higher glycerol concentration.
出处
《草食家畜》
2009年第4期43-46,共4页
Grass-Feeding Livestock
基金
云南省技术创新人才培养计划(2008PY091)
