摘要
从海南文昌采集23种红树植物的根际土壤,采用GA、HV作为选择性分离培养基,添加复合维生素、放线菌酮、制霉菌素和重铬酸钾,结合适当的预处理:干热120°C60min或干热100°C60min及1%氯胺-T处理30min,平板稀释涂布法(10-1、10-2、10-3)分离其中的小双孢菌。共分离得到199株放线菌,通过培养特征及显微形态观察,发现其中有链霉菌147株、非链霉菌52株。选择7株链霉菌和28株非链霉菌进行了16SrRNA基因序列分析,结果显示19株菌属于小双孢菌属,7株属于链霉菌属,4株属于野野村菌属,2株属于小单孢菌属,1株属于链孢囊菌属,1株属于阿萨诺氏菌属,1株属于小单孢菌科。结果表明红树林根际土壤中蕴含着丰富的小双孢菌资源。
Abstract： To selectively isolate Microbispora sp. from mangrove, 23 rhizosphere soils of different mangrove plants were collected in Wenchang, Hainan, China. After pretreatments of dry heat （100℃ 60 min or 120℃ 60 min） and treatment with Chloramine-T （1%, 30 min at room temperature） for each sample, 100 μL of the 10^-1-10^-3 dilutions of the resultant suspensions were transferred and spread onto the selective isolation media. GA and HV media were employed as the isolation media that supplemented with 0.5 mg/L multi-vitamin, 50mg/L-100 mg/L K2Cr2O7, 50 mg/L cycloheximide and 50 rng/L nystatin. One hundred and ninety nine actinomycetes including 147 Streptomyees-like isolates and 52 non-Streptomyces-like isolates were isolated according to their morphology on agar plates and under microscope. Seven Streptomyces-like isolates and 28 non-Streptomyces-like isolates were putatively identified to genus level based on 16S rRNA gene sequence analysis, which belong to Microbispora（19）, Streptomyces（7）, Nonomuraea（4）, Micromono- spora（2）, Streptosporangium（1）, Asanoa（1）, and one uncertain genus under Micromonosporaceae. It is found that plenty of Microbispora spp. inhabit in mangrove rhizoshere soil.
出处
《微生物学通报》
CAS
CSCD
北大核心
2009年第9期1299-1304,共6页
Microbiology China
基金
国家863计划项目(No.2007AA09Z415)
国家自然科学基金广东联合基金重点项目(No.U0633008)
关键词
红树植物
根际土壤
分离
小双孢菌
Mangrove plant, Rhizosphere soil, Isolation, Microbispora
