摘要
[目的]研究金钗石斛多糖对脂多糖(LPS)诱导的小鼠腹腔巨噬细胞分泌肿瘤坏死因子-α(TNF-α)、一氧化氮(NO)的影响。[方法]用不同浓度金钗石斛多糖作用于小鼠腹腔巨噬细胞,LPS作为诱导剂,采用放射免疫法检测细胞培养液中TNF-α的含量,硝酸酶还原法测定细胞培养液中NO的含量,荧光法测定细胞内诱导型一氧化氮合酶(iNOS)的活性,实时PCR(real-time PCR)法测定TNF-αmR-NA、iNOS mRNA的表达。[结果]与LPS组比较,金钗石斛多糖在50～400mg/L范围内可干预LPS对小鼠巨噬细胞的作用,使小鼠腹腔巨噬细胞TNF-α、NO合成减少,iNOS活性降低,TNF-αmRNA、iNOS mRNA的表达降低。[结论]金钗石斛多糖可改善LPS对小鼠腹腔巨噬细胞的作用,使TNF-αmRNA、iNOS mRNA的表达降低,TNF-α、NO合成减少,从而起到抗炎的作用。
[ Objective ] Study on the effects of Dendribum nobile polysaccharide on tumor necrosis factor-α （TNF-α） and nitric oxide （NO） release by mouse peritoneal macrophage induced Lipopolysaccharide （LPS） in vitro. [ Method] The peritoneal macrophages of mice were conducted with LPS in the presence of Dendribum nobile polysaceharidc at various concentration . TNF-α was detected by radioimmunoassay, NO content of cell culture fluid was determined by nitrate reductase method,the activity of inducible nitric oxide synthase （iNOS） was measured by fluorimetry assay . The expression of TNF-α and iNOS mRNA were observed by real-time polymerase chain reaction （real-time PCR）. [ Result ] Compared with the control group,Polysaccharides of Dendribum nobile could markedly inhibit the secretion of TNF-α and NO and decrease the activity of iNOS and down-regulate the expression of TNF-α m RNA and iNOS mRNA in a dose dependent manner. [ Conclusion ] Dendribum nobile polysaccharide can improve TNF-α mRNA,iNOS mRNA and its protein expression ipduced by Lipopolysaccharide in mouse peritoneal macrophage that may play a very important role in the prevention of inflammation.
出处
《安徽农业科学》
CAS
北大核心
2009年第28期13634-13635,13672,共3页
Journal of Anhui Agricultural Sciences
基金
贵州省中医管理局立项项目
