摘要
目的观察不同浓度睾酮对肿瘤坏死因子-α(TNF-α)作用于人脐静脉内皮细胞(human umbilical vein endothelial cells)ECV-304表达血红素加氧酶-1(HO-1)的影响,初步探讨其作用机制。方法用ELISA法观察不同浓度睾酮及雄激素受体拮抗剂Flutimide、雌激素受体拮抗剂ICI182,780对ECV-304表达HO-1干预作用。结果睾酮在3×10-6,3×10-7,3×10-8,3×10-9mol/L几种浓度下均具有促进TNF-α诱导的ECV-304细胞表达HO-1的量。与3.0×10-8mol/L睾酮干预组相比,予1.0×10-6mol/LICI182,780预处理,ECV-304细胞释放的HO-1的量明显减少,并诱导HO-1的表达呈时间(当t=24h达到最理想的分泌量)、浓度依赖关系。结论睾酮对TNF-α诱导的ECV-304细胞表达HO-1的影响与其浓度有关,生理浓度睾酮对其具有一定的促进作用,而且这种生物学效应是部分通过转化为雌激素后,作用于雌激素受体实现。
Objective To investigate the effects of testosterone of different concentrations on the process of tumor necrosis factor-ix （TNF-α） influencing the releasing of hemeoxygenase-1 （ HO-1 ） in human umbilical vein endothelial cells ECV-304. Methods The effects of testosterone of different concentrations with or without its antagonist Flutamide or estrogen receptors antagonist ICI182, 780 on the senescence of ECV-304 induced by TNF-αwere studied by ELISA quality kit according to the manufacturer instructions strictly. Results Testosterone at low concentrations （3 ×10^-9, 3 ×10^-8 and 3 ×10^-7 mol/L） had the trend of promoting the releasing of HO-1 in ECV-304 induced by TNF-α, most obviously at the concentration of 3 ×10^-8 tool/L（ but P 〉 0. 05 vs TNF-αstimulated group） , whereas higher concentration （3 ×10^-6 mol/L） had stimulated effect on the senescence induced by TNF-α （6. 108 ± 1. 154, P 〈 0. 05 vs TNF-α）. The estrogen receptors antagonist ICI182, 170 rather than Flutamide at concentration of 1 ×10^-6mol/L inhibited the protection effect induced by testosterone at concentration of 3 ×10^-8 mol/L. Dose-and time （with optimal secretion occurring at 24h ）-dependent effect of TNF-αinduced the secretion of HO-1. Conclusions Testosterone modulates the senescence of ECV-304 induced by TNF-α a dose-related manner. And at physiological concentrations, testosterone has the trend of protecting it, mainly by transforming to estrogen acting on estrogen receptor.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2009年第17期2210-2212,共3页
Chinese Journal of Gerontology
基金
全军医药卫生"十一五"计划科技攻关课题(06G144)
