摘要
目的研究载脂蛋白ＡＩ（ａｐｏＡＩ）基因表达调控对血浆高密度脂蛋白（ＨＤＬ）代谢的影响。方法采用已建立的含小鼠金属硫蛋白Ｉ（ＭＴ－Ｉ）启动子的人ａｐｏＡＩ转基因Ｃ５７ＢＬ／６小鼠系，通过Ｓｏｕｔｈｅｒｎ及Ｎｏｒｔｈｅｒｎ杂交技术检测锌诱导前后人ａｐｏＡＩ基因整合和表达的情况。结果转基因小鼠整合４～１５拷贝的人ａｐｏＡＩ基因。人ａｐｏＡＩ基因主要在肝和肾中表达，经重金属锌诱导后，人ａｐｏＡＩ基因可在肝、小肠及肾中高效表达。转基因鼠血浆总ａｐｏＡＩ水平比对照组明显增高，其中人ａｐｏＡＩ水平经锌诱导后增高约４６％。与对照组相比，血浆ＨＤＬ水平在锌诱导前后分别增高约４７％和１０３％。转基因鼠ＨＤＬ和人ａｐｏＡＩ水平间呈显著正相关（ｒ＝０．８５，Ｐ＜０．０１）。结论ＡｐｏＡＩ对血浆ＨＤＬ水平升高有重要的调节作用。
Objective To study the regulation of human apolipoprotein AI(h apo AI) gene expression and its role in high density lipoprotein (HDL) metabolism. Methods Transgenic C57BL/6 mice established in this laboratory with human apoAI gene containing mouse metallothionein I(MT I)promoter were used for investigation. Results Southern blot identified the presence of 4～15 copies of h apo AI gene in the transgenic mice. Northern blot showed that human apo AI mRNA was expressed mainly in the liver and kidneys, and the high level of h apo AI mRNA was obtained in liver, kidneys and small intestine after Zinc(Zn) induction. Total Plasma apoAI Level in transgenic mice was significantly increased than that in the controls, and a high h apoAI level was detected in the transgenic mice (46% increased after Zn induction). Additionally, total and HDL cholesterol levels were noticed to be highly increased to 47% before and 103% after Zn induction in the transgenic mice comparing to that of the controls. The plasma HDL and h apoAI levels were significantly correlated ( r =0 85, P <0 01) in the transgenic mice. Conclusion Apo AI has a profound effect on regulating HDL levels in the transgenic mice. This animal model is considered appropriate for studying the effect of apoAI on lipid metabolism and the mechanism combating atherogenesis.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1998年第7期531-533,共3页
National Medical Journal of China
基金
国家教育委员会高等学校博士点专项科研基金
