摘要
目的从孕妇血循环中分选出有核红细胞（ＮＲＢＣｓ），并证实其是胎儿来源的。方法选用红细胞特异单克隆抗体ＧｌｙｃｏｐｈｏｒｉｎＡ（ＧＰＡ）结合流式细胞术成功地从３例妊娠１６～２４周的孕妇血中分离出ＮＲＢＣｓ，并用Ｙ－染色体特异重复序列ＤＮＡ探针（ＰＹ３．４）荧光原位杂交及聚合酶链反应技术检测Ｙ－染色体特异序列（ＳＲＹ）结果ＦＩＳＨ结果，３例分选出的ＧＰＡ阳性细胞中ＰＹ３．４杂交阳性率分别是３１．４％、０、３８％；其ＰＣＲ结果，分别为阳性、阴性、阳性，并在引产后得到性别证实。结论孕妇外周血中确实存在胎儿有核红细胞。
Objective To isolate fetal nucleated red blood cells (NRBCs) from maternal blood and to confirm its fetal origin.Methods Monoclonal antibody against the glycophorin A (GPA) was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y special probe (PY3.4) repeated sequences (SRY) in maternal blood from women carrying male fetuses were detected by polymerase chain reaction (PCR).Results The PY3.4 position hybridization rates were 31.5%,0 and 38% respectively in sorted GPA positive cells (GPA +) of 3 samples by FISH,and the results of GPA + cells of the 3 cases by PCR were positive,negative and positive respectively,which were manifested by the gender after aborticide. Conclusion This study demonstrates that fetal NRBCs are present in the peripheral blood of pregnant women and it is possible to isolate and detect them.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
1998年第2期111-114,共4页
Chinese Journal of Medical Genetics
基金
甘肃省自然科学基金
关键词
胎儿
有核红细胞
流式细胞术
荧光原位杂交
Fetal nucleated erythrocytes Flow cytometry Fluorescence in situ hybridization
