摘要
本研究利用PCR技术和地高辛标记的DNA探针对59例早孕绒毛组织中的沙眼衣原体感染进行研究。结果发现2例绒毛组织有沙眼衣原体感染，其感染率为3．4％，研究证实沙眼衣原体可引起孕早期的胎儿宫内感染。建议对沙眼衣原体感染的孕妇进行宫内感染的产前诊断。
Chlamydia trachomatis is the most commoninfection in human reproduction today. This infection can lead to sterility and other complication during pregnancy. But It's still not clear on the intrauterine infeciton of C. trachomatis. The chorionic villous sampling of the first trimester of pregnancy and the techniques of molecular biology were used to detect C. trachomatis infection in the early pregnancy. We collected 59 chorionic villous samples, and dedtected C. trachomatis by using the method of polymerase chain reaction (PCR). The products of PCR were hybridized with a special probe of C. trachomatis labeled with the DIG system (Boehrringer). The reslult showed 2 positive sample of chorionic villi of C. trachomatis infection in 59 speciments. We suppose that C. trachomatis could lead intrauterine infection in the early pregnancy. PCR combined with DIG dot blot may be a sensitive method for detecting C. trachomatis infection. We suggest that these methods could be used to do prenatal diagnosis of C. trachomatis intrauterine infection in the early pregnancy.
出处
《中国优生与遗传杂志》
1997年第2期17-17,11,共2页
Chinese Journal of Birth Health & Heredity
基金
国家计划生育委员会科研经费的支持
关键词
沙眼衣原体
宫内感染
聚合酶链反应
Chlamydia trachomatic, Intrauterine infection, Polymerase chain reaction,Dot blot
