摘要
从吡啶－２－乙醛苯甲酰腙作柱前衍生试剂，在优化了的衍生和色谱条件下，实现了用高效液相色谱法同时测定铅和汞离子。在ｐｈｅ－ｎｏｍｅｎｅｘｓｐｈｅｒｅｘＣ１８色谱柱上，用甲醇－水（６５∶３５，Ｖ／Ｖ）作流动相，于２４８ｎｍ处检测，得到线性良好的工作曲线，铅和汞的最低检测限分别为２．５５μｇ／Ｌ和７．２９μｇ／Ｌ。分析了环境水样品和合成水样，铅和汞的标准加入回收率分别为９８．３％～１０１．７％和９８．０～１０２．０％。
new method was proposed for the quantitcation of lead and mercury anion by HPLC after derivatization with pyridine 2 acetaldehyde benzoylhydrazone.Some improvements in the conditions for the derivatization and separation were made using methanol water as the mobile phase at a flow rate of 1.0mL/min .The derivatives were separated on Spherex C 18 column and detected at 348nm.The minimum detection amounts for Pb 2+ and Hg 2+ were 2.55μg/L and 7.29ng/mL repectionely .The method was used to determine lead and mercury in water samples with satisfactory results.The recoveries for Pb 2+ and Hg 2+ were 98.3%～102% and 98%～102%, reapectively
出处
《分析试验室》
CAS
CSCD
1998年第4期70-72,共3页
Chinese Journal of Analysis Laboratory
关键词
高效液相色谱
吡啶
乙醛苯甲酰腙
铅
汞
测定
High performance liquid chromatography
pyridine 2 acetaldehyde benzoylhydrazone
lead
mercury
