摘要
[目的]为了探明鳙鳃凝集素在各种条件下的稳定性及其细胞凝集活性。[方法]测定经各种条件处理过的鳙鳃凝集素样品的血凝活性,评价其稳定性;通过镜检测定鳙鳃凝集素对微藻细胞及各种微生物细胞的凝集作用。[结果]琥珀酰化修饰、胰蛋白酶水解、SDS及β-巯基乙醇处理均使鳙鳃凝集素活性大大降低;在设定的处理浓度下,二甲基亚砜、NaC l及三氟乙酸对其活性没有影响;鳙鳃凝集素能专一地凝集雨生红球藻及哈维氏弧菌和鳗弧菌。[结论]鳙鳃凝集素稳定性受各种因素影响,能够选择性地凝集雨生红球藻及2株鱼类病原弧菌。
[Object] To investigate the stability of lectin from gill of bighead carp under various conditions and cell agglutinating activity. [ Method ] Hemagglutination activity of lectin samples after treated under different condition was tested, and its stability was evaluated ; and ag- glutinating effect to the microalgae cells and microorganism were observed by an inverted microscope. [ Result] sueeinylation modified,trypsin hydrolysis, SDS and [^-mercaptoethanol treatment greatly reduced leetin from gill of bighead carp; dimethyl sulfoxide, NaC1, tri fluoroaeetic acid at a given treated concentration had no effect on its activity; leetin from gill of bighead carp agglutinated Haenateoccus pluvialis, vihrio harveyi and Vibrioauguil larum specifically. [ Conclusion ] The stability of lectin from gill of bighead carp had been effected by various factors, and can agglutinate Haenatcoceus pluvialis and two strain fish pathogenic vibrio selectively.
出处
《安徽农业科学》
CAS
北大核心
2009年第26期12589-12591,共3页
Journal of Anhui Agricultural Sciences
关键词
凝集素
稳定性
细胞凝集
血凝活性
Lectin
Stability
Cell agglutination
Hemagglutinating activity
