摘要
目的通过丹酚酸B眼用凝胶滴眼作用于糖尿病大鼠白内障眼,观察大鼠晶状体的变化,以探讨实验性糖尿病大鼠白内障眼药物治疗的可能性。方法经大鼠阴茎背静脉注射链脲佐菌素(streptozotocin,STZ)制备糖尿病大鼠白内障眼。实验分为空白对照组20只(A组)、STZ模型组30只(B组)和丹酚酸B眼用凝胶滴眼组30只(C组),每周裂隙灯显微镜照相机检查记录晶状体变化。于6个月后实验结束时处死大鼠,A、B、C组大鼠各有20只、18只、21只存活。取晶状体检测超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathion peroxidase,GSH-PX)和丙二醛(malondialdehyde,MDA)含量。结果裂隙灯观察B、C组大鼠晶状体的混浊程度明显比A组加重(χA2C=7.746,0.025>P>0.01;χ2AB=48.56,P<0.005),而C组大鼠晶状体的混浊程度明显比B组减轻(χB2C=18.85,P<0.005)。B组晶状体中MDA含量(0.65±0.10)nmol.mg-1较A组(0.12±0.04)nmol.mg-1显著升高(P<0.05);而SOD(30.35±4.17)U.mg-1、GSH-PX(1.28±0.08)U.mg-1活性较A组SOD(55.53±9.98)U.mg-1、GSH-PX(2.07±0.14)U.mg-1的活性显著下降(P均<0.05)。C组MDA含量(0.16±0.09)nmol.mg-1与B组相比较低(P<0.05),SOD(50.48±5.17)U.mg-1、GSH-PX(1.76±0.11)U.mg-1活性均高于B组(P均<0.05)。C组与A组比较MDA含量、SOD、GSH-PX活性差异均无统计学意义(P均>0.05)。结论本组丹酚酸B眼用凝胶滴眼可减轻糖尿病大鼠晶状体的混浊程度,可能与丹酚酸B通过抗脂质过氧化作用,提高细胞耐缺氧能力,抑制氧自由基的大量生成,来保护晶状体上皮细胞有关。
Objective To explore the possibility of drug therapy for experimental diabetic cataract and observe the changes of lens through dropping ophthalmic gel of salvianolic acid B for diabetic cataract eyes in rats. Methods Diabetic cataract eyes of rats were made by injecting streptozotoein through rats＇ dorsal venous of penis. All rats were divided into 3 groups, group A（ control group, 20 rats ）, group B （ streptozotocin model group, 30 rats） and group C （ ophthalmic gel of salvianolic acid B group, 30 rats） Changes of lens were recorded weekly by using slit lamp microscope camera. Six months later,there were 20 rats,18 rats,21 rats survived in group A,group B,and group C, respectively, and they all were killed when the experiment was over. Concentrations of superoxide dismutase （ SOD）, glutathion peroxidase （GSH-PX） and malondialdehyde （MDA） in lens were detected. Results Turbid level of lens in group B and group C were significantly higher than that in group A with slit lamp microscope （χ^2AC = 7. 746, 0. 025 〉 P 〉 0.01 ;χ^2AB = 48.56 ,P 〈 0.005 ）, and the turbid level in group C was much lower than that in group B（χ^2BC = 18.85,P 〈0. 005）. In group B,the level of MDA（0.65 ± 0. 10）nmol ·mg^-1 was higher than that of group A（0. 12 ±0.04） nmol · mg^-1 （P〈 0. 05 ） ,while the activites of SOD（30.35 ±4.17）U · mg^-1 and GSH-PX（ 1.28 ±0.08）U · mg^-1 were significantly decreased compared with SOD （ 55.53 ± 9.98 ）U · mg^-1 and GSH-PX（ 2.07 ± 0.14 ）U · mg^-1in group A（ both P 〈 0.05 ）. Compared with group B, the level of MDA（0. 16 ±0.09） nmol · mg^-1 was lower,while the activities of SOD（50. 48 ± 5.17）U · mg^-1 and GSH-PX（ 1.76 ± 0. 11 ）U · mg^-1 were increased in group C（ all P 〈 0.05 ）. There was no statistical difference in the level of MDA, and the activities of SOD and GSH-PX between group C and group A（ all P 〉 0.05 ）. Conclusions Ophthalmic gel of salvianolic acid B can reduce the turbid level of lens in diabetic rats, which may have relationships with enhancing anti-hypoxia ability of cells, inhibiting the production of oxygen-derived free radicals through its anti-lipid peroxidation, so as to protect lens epithelial cells.
出处
《眼科新进展》
CAS
北大核心
2009年第9期674-676,共3页
Recent Advances in Ophthalmology
基金
黑龙江省自然科学基金资助(编号:D2007-51)
黑龙江省卫生厅科研基金资助(编号:2007-225)
黑龙江省教育厅科研基金资助(编号:11531087)~~
关键词
丹酚酸B眼用凝胶
糖尿病
白内障
大鼠
ophthalmic gel of salvianolic acid B
diabetes mellitus
cataract
rat
