摘要
目的体外高效扩增肿瘤浸润淋巴细胞（ＴＩＬ），为进一步开展基因治疗肿瘤奠定基础。②方法采用机械分离、酶消化和不连续密度梯度离心法分离提取ＴＩＬ前体细胞；以ＩＬ－２作为诱导剂，采用液相三步扩增方法扩增培养，并分别用ＭＴＴ和ＩＦＡ法与对照的自体ＬＡＫ细胞比较细胞动力学。同时对Ｋ５６２细胞和自体肿瘤细胞（ＡＴＣ）进行杀伤试验以及表型检测。③结果从２８例实体瘤组织和肿瘤引流淋巴结中获取的ＴＩＬ前体细胞，２６例培养成功，其中２３例ＴＩＬ数量达到治疗数量级；ＴＩＬ细胞对ＡＴＣ杀伤作用强于自体ＬＡＫ细胞（ｔ＝１１．４，１８．９，Ｐ＜０．０１）。ＴＩＬ细胞表型特点为ＣＤ３，ＣＤ４，ＣＤ８淋巴细胞亚群。④结论ＴＩＬ细胞具有高效杀肿瘤细胞作用，ＴＩＬ细胞可作为基因治疗的理想载体细胞。
Objective To amplify tumor infiltrating lymphocyte(TIL) so as to provide scientific data for gene therapy in the future. Methods TIL were obtained through mechanical separation, enzyme digestion and discontinuous density gradient centrifugation. The cells were amplified by liquid three-step method in the presence of IL-2. Cell dynamics were determined with MTT and IFA methods and compared with autoLAK cells. The killing test and phenotype determination were done for K562 cells and autotumor cells(ATC). Results TIL were successfully cultured in 26 out of 28 samples obtained from tumor tissues or tumor draining lymph nodes(TDLN), of which 23 samples reached the number of therapy grade. The killing activity of TIL for ATC was greater than that of autoLAK cells(t=11.4—18.9, P<0.01). The phenotype of TIL were CD_3,CD_4 and CD_8. ConclusionTIL had strong tumor cell killing activity and could be used as carrier for gene therapy in the future.
出处
《青岛医学院学报》
1998年第3期166-168,共3页
Acta Academiae Medicinae Qingdao Universitatis
基金
山东省卫生厅青年科学基金
关键词
淋巴细胞
肿瘤润润
肿瘤
液相三步扩增法
lymphocytes, tumor infiltrating
cytotoxic activity test, immune
liquid three-step amplification
