摘要
目的体外扩增经ＣＤ３单抗（ＣＤ３ＭｃＡｂ）活化的杀伤细胞（ＣＤ３ＡＫ），并测定其抗肿瘤细胞作用和表型变化。②方法取１３例肺癌和２例肝癌病人外周血，经密度梯度离心获取淋巴细胞，以ＣＤ３ＭｃＡｂ和ＩＬ－２作为诱导剂，采用液相三步扩增方法扩增培养，并分别用ＭＴＴ和ＩＦＡ法与自体ＬＡＫ对照细胞比较细胞动力学、细胞毒作用和表型变化。③结果ＣＤ３ＡＫ细胞扩增速度、数量和抗肿瘤活性均优于自体ＬＡＫ细胞，其表型为ＣＤ３，ＣＤ４，ＣＤ８和ＣＤ１６的特殊异源淋巴细胞群。其中ＣＤ４亚群表型数量随培养时间延长明显升高（χ２＝７．６６，Ｐ＜０．０１），而ＣＤ１６则明显下降（χ２＝５．２３，Ｐ＜０．０５），该亚群表型变化可能与培养时间有关。④结论ＣＤ３ＡＫ细胞具有较强的杀肿瘤细胞作用，同时也可作为基因治疗的理想载体细胞。
Objective To amplify CD_3AK cells in vitro, and check their activity of killing tumor cells and the change of lymphocytic phenotype. Methods The peripheral blood was obtained from 13 pulmonary carcinoma and 2 liver cancer patients. Lymphocyte was separated by density gradient centrifugation, and suspended in medium with CD_3McAb and IL-2 as inducing agents. The cells were proliferated by a special method of liquid threestep amplifications. The cellular dynamic, cytotoxic activity and lymphocyte phenotype were studied by MTT and IFA methods and compared with autoLAK cells. Results The proliferation speed and the number of CD_3AK cells and their antitumor activity were significantly higher than those of autoLAK cells. The phenotypes of CD_3, CD_4, CD_8 and CD_16 were special subpopulations of heterogenous lymphocytes, among which the subtype CD4, increased significantly along with the prolongation of culture time(χ2=7.66,P<0.01), while subtype CD_16 decreased significantly(χ2=5.23,P<0.05). Conclusion CD3AK cells had strong antitumor activity and could be used as ideal carrier for gene therapy in the future.
出处
《青岛医学院学报》
1998年第3期163-165,共3页
Acta Academiae Medicinae Qingdao Universitatis
基金
山东省卫生厅青年科学基金
关键词
细胞毒实验
CD3AK细胞
肿瘤
细胞培养
iller cells, lymphokine activity
lymphocytic subpopulations
interleukin-2
cytotoxic activity test,immune
