摘要
目的：探讨重症肌无力（ＭＧ）抗乙酰胆碱受体抗体（ＡｃｈＲａｂ）、抗突触前膜受体抗体（ＰｓｍＲａｂ）和Ｔ淋巴细胞亚群与临床的关系。方法：应用ＥＬＩＳＡ法检测４０例重症肌无力病人血清中上述两种自身抗体，应用Ｔ淋巴细胞亚群单克隆抗体微量细胞毒法检测该组病人Ｔ淋巴细胞亚群。结果：①ＡｃｈＲａｂＩｇＧ型阳性２４例（６０％）、ＩｇＭ型阳性１５例（３７．５％），ＰｓｍＲａｂＩｇＧ型阳性２４例（６０％）、ＩｇＭ型阳性１１例（２７．５％）；②仅ＡｃｈＲａｂＩｇＧ型阳性３例（７．５％）、ＩｇＭ型阳性１例（２．５％），只有ＰｓｍＲａｂＩｇＧ型阳性２例（５％），二种抗体的ＩｇＧ型和ＩｇＭ型均阴性７例（１７．５％）；③ＡｃｈＲａｂ与ＰｓｍＲａｂ存在直线正相关；④本组病人ＣＤ＋４增高、ＣＤ＋８降低、ＣＤ＋４／ＣＤ＋８比率增高；⑤ＣＤ＋４／ＣＤ＋８比率与这两种自身抗体无相关关系；⑥年龄大于４０岁组的ＣＤ＋８低于４０岁以下组，而ＣＤ＋４／ＣＤ＋８高于４０岁以下年龄组；⑦病程大于或等于３年组的ＣＤ＋８、ＣＤ＋４／ＣＤ＋８分别低于、高于病程小于３年组。结论：ＭＧ病人存在体液免疫和细胞免疫的异常；ＭＧ可受到ＡｃｈＲａｂ和ＰｓｍＲａｂ的免疫性损害?
Objective: To study the relationship between anti acetylcholine receptor(AchR) antibodies(AchRab), antipresynaptic membrane receptor antibodies (PsmRab), and peripheral blood T cell subsets in myasthenia gravis (MG) Methods: AchR and presynaptic membrane receptor were purified by α bungarotoxin and β bungarotoxin as antigens, Forty serum samples of MG were determimed by ELISA, and peripheral blood T cell subsets in those patients were determined by monoclonal anti T cell antibodies Rasults:(1)AchRab positive of IgG type was found in 24 patients (60%) and IgM type in 15 (37 5%). PsmRab positive of IgG type was foune in 24 (60%) and IgM Type in 11 (27 5%);(2) Only AchRab IgG type positive was found in 3 cases (7 5%) and IgM type positive in 1 case (2 5%); Only PsmRab IgG type positive was in 2 cases (5%);Both AchRab and PsmRab (IgG and IgM) negative was found in 7 cases (17 5%); (3) There was positive correlation between AchRab and PsmRab; (4)CD + 4 cell and CD + 4/CD + 8 ratio were higher in the patients than in the controls; (5) CD + 8 cell was lower in the patients than in the controls; (6) CD 8 +,、CD 4 +/CD 8 + ratio differed in different groups of duration and age; (7) There was no correlation between T cell subsets with AchRab and those with PsmRab Conclusions:(1) MG may be injuried by AchRab and PsmRab, or either of them; (2)CD + 4/CD + 8 may better reflect the abnormality of cellular immunity; (3) Abnormality of cellular immunity is more serious in the group of longer duration (≥3 years) and in the group of older age (≥40 years old)
出处
《广西医科大学学报》
CAS
1998年第2期20-23,共4页
Journal of Guangxi Medical University
关键词
重症肌无力
T细胞亚群
ACHRAB
RsmRab
myasthenia gravis
anti acetylcholine receptor
antipresynaptic membrane receptor antibody
T cell subsets
