摘要
采用正交设计对甜菜的RAPD反应体系进行优化.应用L25(56)正交表,研究了Taq、Mg2+、随机引物、dNTPs和DNA模板5种RAPD反应组分浓度变化对扩增结果的影响,并进行量化分析,试验结果表明用这种方法建立的甜菜RAPD-PCR优化反应体系为:25μL反应体系中含1×Buffer、1.0mmol/LMg2+、1UTaqDNA聚合酶、0.25mmol/LdNTPs、0.4μmol/L随机引物及25ngDNA模板.
The L25 （56） orthogonal diagram was applied to study the effects of different concentrations of Taqpolymerase, Mg^2＋, dNTPs, primer and DNA on sugar beet. The results showed that the orthogonal design could be used to optimize the RAPD-PCR system. A suitable RAPD reaction system for Sugar beet was developed, 1 ×Taq polymerase reaction buffer, 1.0mmol/L Mg^2＋, 1U Taq DNA polymerase, 0.25mmol/L dNTPs, 0.4μmol/L primer and 25ng genomic DNA template with total 25μL reaction solution..
出处
《伊犁师范学院学报（自然科学版）》
2009年第3期24-30,共7页
Journal of Yili Normal University：Natural Science Edition
关键词
甜菜
RAPD
反应体系
正交设计
sugar beet
RAPD
Reaction system
Orthogonal design
