摘要
目的了解湖州市老年结核病结核分枝杆菌基因型分布特征,发现在湖州市老年患者中的结核菌主要流行株。方法应用VNTR分型技术,检测结核分枝杆菌基因组中具有多态性的13个位点。通过聚类分析对菌株进行基因分型。结果65例老年结核病患者中,结核分枝杆菌共分4型,其中Ⅰ型占64.6%,Ⅱ型占15.4%,Ⅲ型占3.1%,Ⅳ型占16.9%。结论Ⅰ型结核分枝杆菌菌株为湖州市老年结核病患者的主要流行株。
Objective To investigate the genotypic distribution and the predominant isolates of Mycobacterium tuberculosis in the elderly in Huzhou City. Methods Variable number tandem repeat （VNTR） typing was used to detect thirteen tandem repeat loci. The characters of the genotype were analyzed with SPSS 13.0 software. Results Sixty-five Mycobacterium tuberculosis isolates were classified into four genotype clusters （type Ⅰ , type Ⅰ , type Ⅲ, type Ⅳ） by 13 loci of VNTRs. Most isolates belonged to type Ⅰ , Ⅳ, Ⅲ , which .accounted for 64.6%, 16. 9% and 15.4%, respectively. While type Ⅲ only accounted for 3.1%. Conclusion Type Ⅰ is the epidemic strain cluster of Mycobacteriurn tuberculosis in the elderly in Huzhou City.
出处
《中国预防医学杂志》
CAS
2009年第8期756-758,共3页
Chinese Preventive Medicine
关键词
结核分枝杆菌
数目可变串联重复序列
老年
Mycobacterium tuberculosis
Variable number tandem repeat （VNTR）
The Elderly
