摘要
目的观察新生鼠窒息后缺氧-缺血-再灌注损伤不同时间脑组织NF-κB动态变化,以探讨NF-κB信号途径在新生儿窒息脑损伤中的作用。方法出生7 d新生鼠随机分为对照组(A组)和窒息组(B组);制备新生鼠窒息模型,并于窒息后1、3、5和7 d取脑组织行石蜡切片。采用免疫组织化学方法检测窒息新生鼠脑组织NF-κB p65活性表达水平,用苏木精-伊红染色观察窒息后脑组织形态学变化。结果B组脑组织NF-κB的表达较A组显著增强(P<0.05),以5 d时升高最明显;并伴有神经细胞坏死及凋亡现象。结论NF-κB可能通过炎症反应机制介导窒息脑损伤过程。
Objective To observe the activation of NF-κB in hypoxia ischemia reperfusion brain injury of newborn rats with asphyxia. The aim of study is to explore the effect of NF-KB signal pathway on brain injury of newborn with asphyxia, so as to provide the experimental base for finding clinical treatment of asphyxia,in which NF-KB is taken as the target. Methods newborn rats （ 7 days old） were randomly divided into control group （ A ） and asphyxia group （ B ）. The model of asphyxia was induced in B group. Immunohistochemical method was used to detect the activity of NF-κB P65 in the brain of the newborn rats, and hematoxylin-eosin staining was perform ed to observe the histological evidence of asphyxia in the brain. Results The activity of NF-κB P65 was higher in group B than that in group A （ P 〈 0. 05 ）, increased to the highest peak at 5 d, with nerve cell death and apoptosis in group B. Conclusion The NF-κB, may play an important role in brain injury with asphyxia.
出处
《滨州医学院学报》
2009年第4期269-271,共3页
Journal of Binzhou Medical University
