摘要
研究采用PCR-SSCP技术研究番鸭不同就巢群体(就巢1月群、就巢2月群、就巢3月群)、番鸭非就巢群和白改鸭5个群体250个个体PRL基因第4外显子多态性与就巢性状之间的相关性。结果表明,在外显子4编码区内发现2个SNP位点,分别位于该基因的3777 bp(T/C)和3785 bp(A/G)处。基因型与就巢性状指标相关分析的结果表明,番鸭非就巢群体与各就巢群体间差异极显著(P<0.01),同时番鸭与白改鸭差异显著(P<0.05),推测PRL基因与就巢性有一定的相关。
PCR-SSCP technique was used to analyze the polymorphisms at the e/con 4 of the PRL gene in 250 individuals among five duck populations （non-broodiness museovys, one month broodiness, two months broodiness and three months broodiness muscovies, and Baigai ducks） to further investigate the correlation between polymorphism and broodiness traits. The results showed that there are two SNP sites in the fragment of exon 4, locating at 3 777 bp（T/C） and 3 785 bp （A/G）, respectively. There were significant differences between nonbroodiness muscovies and broodiness muscovies, muscovies and bgducks in genotypes, which indicated that PRL gene may be a candidate gene for the selection of broodiness traits in ducks and a possible molecular marker for production performance of muscovies.
出处
《上海交通大学学报（农业科学版）》
2009年第4期331-334,共4页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
福建省科技厅重大专项资助(2006NZ0003-1)
福建省自然基金资助(2007J0321)
关键词
鸭
就巢
PRL基因
SNP
Muscovy duck
broodiness traits
PRL gene
SNP
